Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles

The aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate repl...

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Autores principales:	SÁNCHEZ,GITTITH, CUELLAR,DANIELA, ZULANTAY,INES, GAJARDO,MARTA, GONZÁLEZ-MARTIN,GUILLERMO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	nifurtimox nanoparticles amastigotes trypomastigotes Trypanosoma cruzi
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100007
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spelling	oai:scielo:S0716-976020020001000072003-01-28Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticlesSÁNCHEZ,GITTITHCUELLAR,DANIELAZULANTAY,INESGAJARDO,MARTAGONZÁLEZ-MARTIN,GUILLERMO nifurtimox nanoparticles amastigotes trypomastigotes Trypanosoma cruzi The aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate replicative intracellular form is amastigote, in vitro studies were performed on this form of parasite as well as on cell culture derived trypomastigotes. The fluorescence method used here was very useful as it allowed for the simultaneous study of trypanocide activity and cytotoxicity by determining living or dead parasites within living or dead host cells. According to these results, the greatest trypanocide activity on cell culture-derived trypomastigotes was recorded for nifurtimox-loaded nanoparticles with a 50% inhibitory concentration (IC50) twenty times less than that of the free drug. The cytotoxycity of unloaded nanoparticles at low concentrations was similar to that obtained by free drug when evaluated on Vero cells. Furthermore, nifurtimox-loaded nanoparticles showed increased trypanocide activity on intracellular amastigotes with an IC50 thirteen times less than that of nifurtimox. We also observed that the unloaded nanoparticles possess the previously-described trypanocide activity, similar to the standard solution of nifurtimox, although the mechanism for this has not yet been elucidated. In conclusion, it was possible to establish in vitro conditions using nifurtimox encapsulated nanoparticles in order to decrease the doses of the drug and thus to obtain high trypanocidal activity on both free trypomastigotes and intracellular amastigotes with low cytotoxicity for the host cell.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.1 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100007en10.4067/S0716-97602002000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	nifurtimox nanoparticles amastigotes trypomastigotes Trypanosoma cruzi
spellingShingle	nifurtimox nanoparticles amastigotes trypomastigotes Trypanosoma cruzi SÁNCHEZ,GITTITH CUELLAR,DANIELA ZULANTAY,INES GAJARDO,MARTA GONZÁLEZ-MARTIN,GUILLERMO Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
description	The aim of the present study was to study the trypanocidal activity of nanoparticles loaded with nifurtimox in comparison with the free drug against Trypanosoma cruzi, responsible for Chagas' disease. Ethylcyanoacrylate nanoparticles acted as the delivery system into cells. As the obligate replicative intracellular form is amastigote, in vitro studies were performed on this form of parasite as well as on cell culture derived trypomastigotes. The fluorescence method used here was very useful as it allowed for the simultaneous study of trypanocide activity and cytotoxicity by determining living or dead parasites within living or dead host cells. According to these results, the greatest trypanocide activity on cell culture-derived trypomastigotes was recorded for nifurtimox-loaded nanoparticles with a 50% inhibitory concentration (IC50) twenty times less than that of the free drug. The cytotoxycity of unloaded nanoparticles at low concentrations was similar to that obtained by free drug when evaluated on Vero cells. Furthermore, nifurtimox-loaded nanoparticles showed increased trypanocide activity on intracellular amastigotes with an IC50 thirteen times less than that of nifurtimox. We also observed that the unloaded nanoparticles possess the previously-described trypanocide activity, similar to the standard solution of nifurtimox, although the mechanism for this has not yet been elucidated. In conclusion, it was possible to establish in vitro conditions using nifurtimox encapsulated nanoparticles in order to decrease the doses of the drug and thus to obtain high trypanocidal activity on both free trypomastigotes and intracellular amastigotes with low cytotoxicity for the host cell.
author	SÁNCHEZ,GITTITH CUELLAR,DANIELA ZULANTAY,INES GAJARDO,MARTA GONZÁLEZ-MARTIN,GUILLERMO
author_facet	SÁNCHEZ,GITTITH CUELLAR,DANIELA ZULANTAY,INES GAJARDO,MARTA GONZÁLEZ-MARTIN,GUILLERMO
author_sort	SÁNCHEZ,GITTITH
title	Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
title_short	Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
title_full	Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
title_fullStr	Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
title_full_unstemmed	Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
title_sort	cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100007
work_keys_str_mv	AT sanchezgittith cytotoxicityandtrypanocidalactivityofnifurtimoxencapsulatedinethylcyanoacrylatenanoparticles AT cuellardaniela cytotoxicityandtrypanocidalactivityofnifurtimoxencapsulatedinethylcyanoacrylatenanoparticles AT zulantayines cytotoxicityandtrypanocidalactivityofnifurtimoxencapsulatedinethylcyanoacrylatenanoparticles AT gajardomarta cytotoxicityandtrypanocidalactivityofnifurtimoxencapsulatedinethylcyanoacrylatenanoparticles AT gonzalezmartinguillermo cytotoxicityandtrypanocidalactivityofnifurtimoxencapsulatedinethylcyanoacrylatenanoparticles
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Cytotoxicity and trypanocidal activity of nifurtimox encapsulated in ethylcyanoacrylate nanoparticles

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