New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse

Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes...

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Autores principales:	FAROUDI,MUSTAPHA, ZARU,ROSSANA, FAVIER,BENOIT, VALITUTTI,SALVATORE
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	T cell antigen receptor Immunological synapse signal transduction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004
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spelling	oai:scielo:S0716-976020020002000042002-12-26New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapseFAROUDI,MUSTAPHAZARU,ROSSANAFAVIER,BENOITVALITUTTI,SALVATORE T cell antigen receptor Immunological synapse T cell antigen receptor signal transduction Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zetacomplex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues: (A) Recruitment and the three-dimensional arrangement of receptors and signaling components at the contact site between human CD4+ T lymphocytes and APCs, (B) Structure of the immunological synapse formed at the contact site between cytotoxic T lymphocytes (CTLs) and target cells. We discuss evidence indicating that TCR engagement and triggering can occur in the absence of large-scale molecular segregation into the T cell-APC contact site. Taken together our results indicate that although not required for TCR engagement and triggering, formation of the IS is important to reinforce TCR-mediated signal transduction and achieve full T cell activation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.2 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004en10.4067/S0716-97602002000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	T cell antigen receptor Immunological synapse T cell antigen receptor signal transduction
spellingShingle	T cell antigen receptor Immunological synapse T cell antigen receptor signal transduction FAROUDI,MUSTAPHA ZARU,ROSSANA FAVIER,BENOIT VALITUTTI,SALVATORE New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
description	Three innovative and complementary morphological approaches were employed to study the T cell/antigen presenting cell (APC) interaction: (i) high resolution three-dimensional confocal microscopy of the T cell-APC contact site; (ii) time lapse video recording in living T cells of [Ca2+]I and changes in distribution of various GFP fusion proteins with TCR/CD3-zetacomplex associated- and other signaling components; (iii) measurement of lateral TCR mobility and that of recruited signaling components using techniques based on fluorescence recovery after photo-bleaching. These approaches were combined with biochemical and functional experiments to investigate two principal issues: (A) Recruitment and the three-dimensional arrangement of receptors and signaling components at the contact site between human CD4+ T lymphocytes and APCs, (B) Structure of the immunological synapse formed at the contact site between cytotoxic T lymphocytes (CTLs) and target cells. We discuss evidence indicating that TCR engagement and triggering can occur in the absence of large-scale molecular segregation into the T cell-APC contact site. Taken together our results indicate that although not required for TCR engagement and triggering, formation of the IS is important to reinforce TCR-mediated signal transduction and achieve full T cell activation.
author	FAROUDI,MUSTAPHA ZARU,ROSSANA FAVIER,BENOIT VALITUTTI,SALVATORE
author_facet	FAROUDI,MUSTAPHA ZARU,ROSSANA FAVIER,BENOIT VALITUTTI,SALVATORE
author_sort	FAROUDI,MUSTAPHA
title	New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
title_short	New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
title_full	New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
title_fullStr	New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
title_full_unstemmed	New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse
title_sort	new insights to the functional role of the t cell-antigen presenting cell immunological synapse
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200004
work_keys_str_mv	AT faroudimustapha newinsightstothefunctionalroleofthetcellantigenpresentingcellimmunologicalsynapse AT zarurossana newinsightstothefunctionalroleofthetcellantigenpresentingcellimmunologicalsynapse AT favierbenoit newinsightstothefunctionalroleofthetcellantigenpresentingcellimmunologicalsynapse AT valituttisalvatore newinsightstothefunctionalroleofthetcellantigenpresentingcellimmunologicalsynapse
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New insights to the functional role of the T cell-Antigen Presenting Cell immunological synapse

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