IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression

Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave a...

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Autores principales:	JAIMOVICH,ENRIQUE, CARRASCO,MARIA ANGELICA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	Excitation-transcription inositol trisphosphate mitogen-activated kinases CREB early genes dihydropyridine receptors
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010
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spelling	oai:scielo:S0716-976020020002000102002-12-26IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expressionJAIMOVICH,ENRIQUECARRASCO,MARIA ANGELICA Excitation-transcription inositol trisphosphate mitogen-activated kinases CREB early genes dihydropyridine receptors Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cellsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.2 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010en10.4067/S0716-97602002000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Excitation-transcription inositol trisphosphate mitogen-activated kinases CREB early genes dihydropyridine receptors
spellingShingle	Excitation-transcription inositol trisphosphate mitogen-activated kinases CREB early genes dihydropyridine receptors JAIMOVICH,ENRIQUE CARRASCO,MARIA ANGELICA IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
description	Potassium depolarization of cultured muscle cells was employed to study cellular responses linked to calcium signaling. Events occurring after depolarization include i) A transient increase of the IP3 mass (2-10s); ii) A slow calcium transient (5 to 25s) that propagates as a low concentration wave along the myotube showing a distinct calcium transient at the level of cell nuclei. Due to the presence of IP3 receptors both in the SR (A-band region) and in the nuclear envelope, these two events appear to be related; iii) Phosphorylation of mitogen activated kinases (ERK 1/2) and of the transcription factor CREB (30 s-10 min), as well as expression of the early genes c-fos, c-jun and egr-1 mRNA (5-15 min). Several independent pieces of evidence, including results obtained using inhibitors specific for individual steps, allowed us to connect these in a sequential manner. As the same type of signaling cascade can be triggered by oxidants, neurotransmitters and hormones, the ensemble of results allows us to propose a general model to describe signaling events that link membrane stimulation to regulation of gene transcription in skeletal muscle cells
author	JAIMOVICH,ENRIQUE CARRASCO,MARIA ANGELICA
author_facet	JAIMOVICH,ENRIQUE CARRASCO,MARIA ANGELICA
author_sort	JAIMOVICH,ENRIQUE
title	IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
title_short	IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
title_full	IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
title_fullStr	IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
title_full_unstemmed	IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression
title_sort	ip3 dependent ca2+ signals in muscle cells are involved in regulation of gene expression
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200010
work_keys_str_mv	AT jaimovichenrique ip3dependentca2signalsinmusclecellsareinvolvedinregulationofgeneexpression AT carrascomariaangelica ip3dependentca2signalsinmusclecellsareinvolvedinregulationofgeneexpression
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IP3 dependent Ca2+ signals in muscle cells are involved in regulation of gene expression

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