Non-selective cation channels and oxidative stress- induced cell swelling

Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transport...

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Autores principales:	SIMON,FELIPE, VARELA,DIEGO, RIVEROS,ANA, EGUIGUREN,ANA LUISA, STUTZIN,ANDRES
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	Cell death Cell Volume Nonselective cation channels Flufenamic acid
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013
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spelling	oai:scielo:S0716-976020020002000132002-12-26Non-selective cation channels and oxidative stress- induced cell swellingSIMON,FELIPEVARELA,DIEGORIVEROS,ANAEGUIGUREN,ANA LUISASTUTZIN,ANDRES Cell death Cell Volume Nonselective cation channels Flufenamic acid Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (<A HREF="#2">Barros et al., 2001a</A>). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for activation and is inhibited by flufenamic acid. Hydrogen peroxide induced a significant increase in cell volume that was dependent on external Na+. We propose that the NSCC, which is ubiquitous though largely inactive in healthy cells, becomes activated under severe oxidative stress. The ensuing Na+ influx initiates via positive feedback a series of metabolic and electrolytic disturbances, resulting in cell death by necrosisinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.2 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013en10.4067/S0716-97602002000200013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cell death Cell Volume Nonselective cation channels Flufenamic acid
spellingShingle	Cell death Cell Volume Nonselective cation channels Flufenamic acid SIMON,FELIPE VARELA,DIEGO RIVEROS,ANA EGUIGUREN,ANA LUISA STUTZIN,ANDRES Non-selective cation channels and oxidative stress- induced cell swelling
description	Necrosis is considered as a non-specific form of cell death that induces tissue inflammation and is preceded by cell swelling. This increase in cell volume has been ascribed mainly to defective outward pumping of Na+ caused by metabolic depletion and/or to increased Na+ influx via membrane transporters. A specific mechanism of swelling and necrosis driven by the influx of Na+ through nonselective cation channels has been recently proposed (<A HREF="#2">Barros et al., 2001a</A>). We have characterized further the properties of the nonselective cation channel (NSCC) in HTC cells. The NSCC shows a conductance of ~18 pS, is equally permeable to Na+ and K+, impermeant to Ca2+, requires high intracellular Ca2+ as well as low intracellular ATP for activation and is inhibited by flufenamic acid. Hydrogen peroxide induced a significant increase in cell volume that was dependent on external Na+. We propose that the NSCC, which is ubiquitous though largely inactive in healthy cells, becomes activated under severe oxidative stress. The ensuing Na+ influx initiates via positive feedback a series of metabolic and electrolytic disturbances, resulting in cell death by necrosis
author	SIMON,FELIPE VARELA,DIEGO RIVEROS,ANA EGUIGUREN,ANA LUISA STUTZIN,ANDRES
author_facet	SIMON,FELIPE VARELA,DIEGO RIVEROS,ANA EGUIGUREN,ANA LUISA STUTZIN,ANDRES
author_sort	SIMON,FELIPE
title	Non-selective cation channels and oxidative stress- induced cell swelling
title_short	Non-selective cation channels and oxidative stress- induced cell swelling
title_full	Non-selective cation channels and oxidative stress- induced cell swelling
title_fullStr	Non-selective cation channels and oxidative stress- induced cell swelling
title_full_unstemmed	Non-selective cation channels and oxidative stress- induced cell swelling
title_sort	non-selective cation channels and oxidative stress- induced cell swelling
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200013
work_keys_str_mv	AT simonfelipe nonselectivecationchannelsandoxidativestressinducedcellswelling AT vareladiego nonselectivecationchannelsandoxidativestressinducedcellswelling AT riverosana nonselectivecationchannelsandoxidativestressinducedcellswelling AT eguigurenanaluisa nonselectivecationchannelsandoxidativestressinducedcellswelling AT stutzinandres nonselectivecationchannelsandoxidativestressinducedcellswelling
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Non-selective cation channels and oxidative stress- induced cell swelling

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