In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA

Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the...

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Autores principales:	RIOS,MARIANA, VENEGAS,ALEJANDRO, CROXATTO,HORACIO B
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	ß-galactosidase gene transfer oviduct
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007
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spelling	oai:scielo:S0716-976020020003000072003-11-26In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNARIOS,MARIANAVENEGAS,ALEJANDROCROXATTO,HORACIO B ß-galactosidase gene transfer oviduct Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal plasmid increased ß-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for ß-gal can enter oviductal cells and be translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNAinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007en10.4067/S0716-97602002000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ß-galactosidase gene transfer oviduct
spellingShingle	ß-galactosidase gene transfer oviduct RIOS,MARIANA VENEGAS,ALEJANDRO CROXATTO,HORACIO B In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
description	Intra-oviductal administration of RNA obtained from oviducts of estradiol-treated rats resulted in accelerated egg transport (<A HREF="#rios97">Ríos et al., 1997</A>). It is probable that estradiol-induced messenger RNA (mRNA) entered oviductal cells and was translated into the proteins involved in accelerated egg transport. In order to test this interpretation we deposited in vivo 50 µg of pure ß-galactosidase (ß-gal) mRNA, 50 µg of pure DNA from the reporter gene ß-gal under SV40 promoter or the vehicle (control oviducts) into the oviductal lumen of rats. Twenty four hours later the ß-gal activity was assayed in oviductal tissue homogenates using o-nitrophenyl-ß-D-galactopyranoside as a substrate. The administration of ß-gal mRNA and pSVBgal plasmid increased ß-gal activity by 71% and 142%, respectively, over the control oviducts. These results indicate that naked DNA and mRNA coding for ß-gal can enter oviductal cells and be translated into an active enzyme. They are consistent with the interpretation that embryo transport acceleration caused by the injection of estradiol-induced RNA in the oviduct involves translation of the injected mRNA
author	RIOS,MARIANA VENEGAS,ALEJANDRO CROXATTO,HORACIO B
author_facet	RIOS,MARIANA VENEGAS,ALEJANDRO CROXATTO,HORACIO B
author_sort	RIOS,MARIANA
title	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
title_short	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
title_full	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
title_fullStr	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
title_full_unstemmed	In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA
title_sort	in vivo expression of ß-galactosidase by rat oviduct exposed to naked dna or messenger rna
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300007
work_keys_str_mv	AT riosmariana invivoexpressionofßgalactosidasebyratoviductexposedtonakeddnaormessengerrna AT venegasalejandro invivoexpressionofßgalactosidasebyratoviductexposedtonakeddnaormessengerrna AT croxattohoraciob invivoexpressionofßgalactosidasebyratoviductexposedtonakeddnaormessengerrna
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In vivo expression of ß-galactosidase by rat oviduct exposed to naked DNA or messenger RNA

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