Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae

The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cere...

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Autores principales:	GÖTTE,MARTIN, STADTBÄUMER,ANDREA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	vesicular transport SNARE syntaxin endosomal trafficking vacuole protein sorting PEP12
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009
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spelling	oai:scielo:S0716-976020020003000092003-11-26Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiaeGÖTTE,MARTINSTADTBÄUMER,ANDREA vesicular transport SNARE syntaxin endosomal trafficking vacuole protein sorting PEP12 The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable of complementing the sorting and maturation defects of the vacuolar hydrolase CPY in pep12 null mutants. No dominant negative effects of either syntaxin 6 or syntaxin 6deltaC overexpression on CPY sorting and maturation were observed in wild-type yeast cells. We conclude that syntaxin 6 and Pep12p do not act at the same vesicular trafficking step(s) in yeast and higher eukaryotesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009en10.4067/S0716-97602002000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	vesicular transport SNARE syntaxin endosomal trafficking vacuole protein sorting PEP12
spellingShingle	vesicular transport SNARE syntaxin endosomal trafficking vacuole protein sorting PEP12 GÖTTE,MARTIN STADTBÄUMER,ANDREA Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
description	The molecular mechanisms of vesicular protein transport in eukaryotic cells are highly conserved. Members of the syntaxin family play a pivotal role in the membrane fusion process. We have expressed rat syntaxin 6 and its cytoplasmic domain in wild-type and pep12 mutant strains of Saccharomyces cerevisiae to elucidate the role of the syntaxin 6-dependent vesicular trafficking step in yeast. Immunofluorescence microscopy revealed a punctate, Golgi-like staining pattern for syntaxin 6, which only partially overlapped with Pep12p in wild-type yeast cells. In contrast to Pep12p, syntaxin 6 was not mislocalized to the vacuole upon expression from 2 micron vectors, which might be attributed to conserved sorting and retention signals. Syntaxin 6 was not capable of complementing the sorting and maturation defects of the vacuolar hydrolase CPY in pep12 null mutants. No dominant negative effects of either syntaxin 6 or syntaxin 6deltaC overexpression on CPY sorting and maturation were observed in wild-type yeast cells. We conclude that syntaxin 6 and Pep12p do not act at the same vesicular trafficking step(s) in yeast and higher eukaryotes
author	GÖTTE,MARTIN STADTBÄUMER,ANDREA
author_facet	GÖTTE,MARTIN STADTBÄUMER,ANDREA
author_sort	GÖTTE,MARTIN
title	Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
title_short	Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
title_full	Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
title_fullStr	Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
title_full_unstemmed	Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae
title_sort	heterologous expression of syntaxin 6 in saccharomyces cerevisiae
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300009
work_keys_str_mv	AT gottemartin heterologousexpressionofsyntaxin6insaccharomycescerevisiae AT stadtbaumerandrea heterologousexpressionofsyntaxin6insaccharomycescerevisiae
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Heterologous Expression of Syntaxin 6 in Saccharomyces cerevisiae

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