Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro

Acetylcholine (ACh), adenosine 5'-triphosphate (ATP) and sodium cyanide (NaCN) activate petrosal ganglion (PG) neurons in vitro, and evoke ventilatory reflexes in situ, which are abolished after bilateral chemosensory denervation. Because in our previous experiments we superfused the isolated P...

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Autores principales:	SOTO,CAROLINA R, ARROYO,JORGE, ALCAYAGA,JULIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	Petrosal Ganglion Carotid sinus nerve Carbon dioxide Bicarbonate Acetylcholine Adenosine 5'- triphosphate Cyanide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300010
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spelling	oai:scielo:S0716-976020020003000102003-11-26Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitroSOTO,CAROLINA RARROYO,JORGEALCAYAGA,JULIO Petrosal Ganglion Carotid sinus nerve Carbon dioxide Bicarbonate Acetylcholine Adenosine 5'- triphosphate Cyanide Acetylcholine (ACh), adenosine 5'-triphosphate (ATP) and sodium cyanide (NaCN) activate petrosal ganglion (PG) neurons in vitro, and evoke ventilatory reflexes in situ, which are abolished after bilateral chemosensory denervation. Because in our previous experiments we superfused the isolated PG with solutions free of CO2 /HCO3¯ buffer, we studied its effects on the PG responses evoked in vitro. PGs from adult cats were superfused at a constant pH, with HEPES-supplemented (5 mM) saline with or without CO2 /HCO3¯ (5% / 26.2 mM) buffer, and carotid (sinus) nerve frequency discharge (CN) recorded. Increases in CN evoked by ACh, ATP and NaCN in CO2-free saline were significantly reduced (P<0.05, Wilcoxon test) when CO2 / HCO3¯ was present in the superfusion medium. Thus, the presence of CO2 / HCO3¯ buffer appears to reduce PG neurons sensitivity to ACh, ATP and NaCN, an effect that may underlie the lack of ventilatory reflexes after bilateral chemodenervationinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300010en10.4067/S0716-97602002000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Petrosal Ganglion Carotid sinus nerve Carbon dioxide Bicarbonate Acetylcholine Adenosine 5'- triphosphate Cyanide
spellingShingle	Petrosal Ganglion Carotid sinus nerve Carbon dioxide Bicarbonate Acetylcholine Adenosine 5'- triphosphate Cyanide SOTO,CAROLINA R ARROYO,JORGE ALCAYAGA,JULIO Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
description	Acetylcholine (ACh), adenosine 5'-triphosphate (ATP) and sodium cyanide (NaCN) activate petrosal ganglion (PG) neurons in vitro, and evoke ventilatory reflexes in situ, which are abolished after bilateral chemosensory denervation. Because in our previous experiments we superfused the isolated PG with solutions free of CO2 /HCO3¯ buffer, we studied its effects on the PG responses evoked in vitro. PGs from adult cats were superfused at a constant pH, with HEPES-supplemented (5 mM) saline with or without CO2 /HCO3¯ (5% / 26.2 mM) buffer, and carotid (sinus) nerve frequency discharge (CN) recorded. Increases in CN evoked by ACh, ATP and NaCN in CO2-free saline were significantly reduced (P<0.05, Wilcoxon test) when CO2 / HCO3¯ was present in the superfusion medium. Thus, the presence of CO2 / HCO3¯ buffer appears to reduce PG neurons sensitivity to ACh, ATP and NaCN, an effect that may underlie the lack of ventilatory reflexes after bilateral chemodenervation
author	SOTO,CAROLINA R ARROYO,JORGE ALCAYAGA,JULIO
author_facet	SOTO,CAROLINA R ARROYO,JORGE ALCAYAGA,JULIO
author_sort	SOTO,CAROLINA R
title	Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
title_short	Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
title_full	Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
title_fullStr	Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
title_full_unstemmed	Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
title_sort	effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300010
work_keys_str_mv	AT sotocarolinar effectsofbicarbonatebufferonacetylcholineadenosine5triphosphateandcyanideinducedresponsesinthecatpetrosalganglioninvitro AT arroyojorge effectsofbicarbonatebufferonacetylcholineadenosine5triphosphateandcyanideinducedresponsesinthecatpetrosalganglioninvitro AT alcayagajulio effectsofbicarbonatebufferonacetylcholineadenosine5triphosphateandcyanideinducedresponsesinthecatpetrosalganglioninvitro
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Effects of bicarbonate buffer on acetylcholine-, adenosine 5´triphosphate-, and cyanide-induced responses in the cat petrosal ganglion in vitro

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