Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases

The development of an effective hypersensitive response (HR) in any plant system relies, not only in their gene composition and expression, but also on an effective and rapid signal transduction system. Lemon seedlings induce the phenylpropanoid pathway, which results in the de novo biosynthesis of...

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Autores principales:	ORTEGA,XIMENA, POLANCO,RUBÉN, CASTAÑEDA,PATRICIA, PEREZ,LUZ M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	Citrus limon hypersensitive response signal transduction
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300012
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spelling	oai:scielo:S0716-976020020003000122003-11-26Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinasesORTEGA,XIMENAPOLANCO,RUBÉNCASTAÑEDA,PATRICIAPEREZ,LUZ M Citrus limon hypersensitive response signal transduction The development of an effective hypersensitive response (HR) in any plant system relies, not only in their gene composition and expression, but also on an effective and rapid signal transduction system. Lemon seedlings induce the phenylpropanoid pathway, which results in the de novo biosynthesis of the phytoalexin scoparone, as part of the hypersensitive response against Alternaria alternata. In order to elucidate some of the signaling elements that participate in the development of HR in lemon seedlings, we used several compounds that are known as activators or inhibitors of signal transduction elements in plants or in animal cells. Lemon seedlings treated either with cholera toxin or with phorbol 12-myristate 13-acetate (PMA), in the absence of A. alternata induced phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone, suggesting the participation of a G-protein and of a serine/threonine kinase, respectively, in signal transduction. The use of trifluoperazine (TFP), W-7, staurosporine, lavendustin A or 2,5dihydroximethyl cinnamate (DHMC) prevented PAL induction as well as scoparone biosynthesis in response to the fungal inoculation, thus allowing us to infer the participation of Calmodulin (CaM), of serine/threonine and of tyrosine protein kinases (TPK) for signal transduction in Citrus limon in response to A. alternatainfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300012en10.4067/S0716-97602002000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Citrus limon hypersensitive response signal transduction
spellingShingle	Citrus limon hypersensitive response signal transduction ORTEGA,XIMENA POLANCO,RUBÉN CASTAÑEDA,PATRICIA PEREZ,LUZ M Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
description	The development of an effective hypersensitive response (HR) in any plant system relies, not only in their gene composition and expression, but also on an effective and rapid signal transduction system. Lemon seedlings induce the phenylpropanoid pathway, which results in the de novo biosynthesis of the phytoalexin scoparone, as part of the hypersensitive response against Alternaria alternata. In order to elucidate some of the signaling elements that participate in the development of HR in lemon seedlings, we used several compounds that are known as activators or inhibitors of signal transduction elements in plants or in animal cells. Lemon seedlings treated either with cholera toxin or with phorbol 12-myristate 13-acetate (PMA), in the absence of A. alternata induced phenylalanine ammonia-lyase (PAL, E. C. 4.3.1.5) and the synthesis of scoparone, suggesting the participation of a G-protein and of a serine/threonine kinase, respectively, in signal transduction. The use of trifluoperazine (TFP), W-7, staurosporine, lavendustin A or 2,5dihydroximethyl cinnamate (DHMC) prevented PAL induction as well as scoparone biosynthesis in response to the fungal inoculation, thus allowing us to infer the participation of Calmodulin (CaM), of serine/threonine and of tyrosine protein kinases (TPK) for signal transduction in Citrus limon in response to A. alternata
author	ORTEGA,XIMENA POLANCO,RUBÉN CASTAÑEDA,PATRICIA PEREZ,LUZ M
author_facet	ORTEGA,XIMENA POLANCO,RUBÉN CASTAÑEDA,PATRICIA PEREZ,LUZ M
author_sort	ORTEGA,XIMENA
title	Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
title_short	Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
title_full	Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
title_fullStr	Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
title_full_unstemmed	Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases
title_sort	signal transduction in lemon seedlings in the hypersensitive response against alternaria alternata: participation of calmodulin, g-protein and protein kinases
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300012
work_keys_str_mv	AT ortegaximena signaltransductioninlemonseedlingsinthehypersensitiveresponseagainstalternariaalternataparticipationofcalmodulingproteinandproteinkinases AT polancoruben signaltransductioninlemonseedlingsinthehypersensitiveresponseagainstalternariaalternataparticipationofcalmodulingproteinandproteinkinases AT castanedapatricia signaltransductioninlemonseedlingsinthehypersensitiveresponseagainstalternariaalternataparticipationofcalmodulingproteinandproteinkinases AT perezluzm signaltransductioninlemonseedlingsinthehypersensitiveresponseagainstalternariaalternataparticipationofcalmodulingproteinandproteinkinases
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Signal transduction in lemon seedlings in the hypersensitive response against Alternaria alternata: participation of calmodulin, G-protein and protein kinases

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