Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak

Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first repo...

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Autores principales:	CÓRDOVA,JOSÉ LUIS, ASTORGA,JOSEFA, SILVA,WALLY, RIQUELME,CARLOS
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2002
Materias:	bacterial bloom bacterial geographical dispersion PCR amplification molecular typification
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017
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spelling	oai:scielo:S0716-976020020003000172003-11-26Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreakCÓRDOVA,JOSÉ LUISASTORGA,JOSEFASILVA,WALLYRIQUELME,CARLOS bacterial bloom bacterial geographical dispersion PCR amplification molecular typification Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first report of this bacterium causing an epidemic in Chile. V. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. These isolates were analyzed by polymerase chain reaction (PCR) amplification of the pR72H gene, a species-specific sequence. Based on the pR72H gene amplification pattern, at least three different isolates of V. parahaemolyticus were found. Two isolates (named amplicons A and C) generated PCR products of approximately 400 bp and 340 bp respectively, while another type of isolate designated B, did not generate a PCR product, regardless of which method of DNA purification was used. Sequence analysis of the amplicons A and C shows that they have an 80 bp and 183 bp conserved region at the 5'end of the gene. However, both isolates have different sequences at their 3' terminus and are also different from the pR72H sequence originally reported. Using this PCR assay we demonstrated that these three isolates were found in clinical samples as well as in shellfish. The warm seawater caused by the climatological phenomena "El Niño" perhaps favored the geographic dispersion of the bacterium (bacterial bloom) occurring in Antofagasta that occurred during that time of yearinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.35 n.3-4 20022002-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017en10.4067/S0716-97602002000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bacterial bloom bacterial geographical dispersion PCR amplification molecular typification
spellingShingle	bacterial bloom bacterial geographical dispersion PCR amplification molecular typification CÓRDOVA,JOSÉ LUIS ASTORGA,JOSEFA SILVA,WALLY RIQUELME,CARLOS Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
description	Between November 1997 and April 1998, several human gastroenteritis cases were reported in Antofagasta, a city in the north of Chile. This outbreak was associated with the consumption of shellfish, and the etiologic agent responsible was identified as Vibrio parahaemolyticus. This was the first report of this bacterium causing an epidemic in Chile. V. parahaemolyticus was the only pathogenic bacterium isolated from patient stools and from shellfish samples. These isolates were analyzed by polymerase chain reaction (PCR) amplification of the pR72H gene, a species-specific sequence. Based on the pR72H gene amplification pattern, at least three different isolates of V. parahaemolyticus were found. Two isolates (named amplicons A and C) generated PCR products of approximately 400 bp and 340 bp respectively, while another type of isolate designated B, did not generate a PCR product, regardless of which method of DNA purification was used. Sequence analysis of the amplicons A and C shows that they have an 80 bp and 183 bp conserved region at the 5'end of the gene. However, both isolates have different sequences at their 3' terminus and are also different from the pR72H sequence originally reported. Using this PCR assay we demonstrated that these three isolates were found in clinical samples as well as in shellfish. The warm seawater caused by the climatological phenomena "El Niño" perhaps favored the geographic dispersion of the bacterium (bacterial bloom) occurring in Antofagasta that occurred during that time of year
author	CÓRDOVA,JOSÉ LUIS ASTORGA,JOSEFA SILVA,WALLY RIQUELME,CARLOS
author_facet	CÓRDOVA,JOSÉ LUIS ASTORGA,JOSEFA SILVA,WALLY RIQUELME,CARLOS
author_sort	CÓRDOVA,JOSÉ LUIS
title	Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
title_short	Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
title_full	Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
title_fullStr	Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
title_full_unstemmed	Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak
title_sort	characterization by pcr of vibrio parahaemolyticus isolates collected during the 1997-1998 chilean outbreak
publisher	Sociedad de Biología de Chile
publishDate	2002
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000300017
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Characterization by PCR of Vibrio parahaemolyticus isolates collected during the 1997-1998 Chilean outbreak

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