Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent

Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble pol...

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Autores principales:	GARCÍA,CARLOS, PEREIRA,PAULO, VALLE,LUIS, LAGOS,NÉSTOR
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Materias:	DSP toxins Okadaic acid Dinophysistoxin-1 (DTX-1) Pyrenyldiazomethane (PDAM) Patagonian fjords Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200007
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spelling	oai:scielo:S0716-976020030002000072003-11-19Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagentGARCÍA,CARLOSPEREIRA,PAULOVALLE,LUISLAGOS,NÉSTOR DSP toxins Okadaic acid Dinophysistoxin-1 (DTX-1) Pyrenyldiazomethane (PDAM) Patagonian fjords Chile Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble polyethers have been known as diarrhetic shellfish toxins. Among them, Okadaic acid (OA), Dinophysistoxin 1 (DTX-1, 35-methyl OA), Dinophysistoxin 2 (DTX-2, OA isomers) and Dinophysistoxin 3 (DTX-3, 7-O-acyl-35-methyl OA), all of which have free carboxilic groups. To perform quantitative analysis of DSP toxins in shellfish samples is a requirement, because DSP toxins are endemic in the Chilean mollusks of the southern regions, and although human symptoms of DSP appear relatively mild in comparison with the Paralytic Shellfish Poisoning (PSP), the necessity of monitoring the chronic effects of continued uptake of low doses of DSP toxins more closely is imperative, since DSP toxins have been described as potent tumor promoters. This paper shows the synthesis pathway of a chromophore, 1-pyrenyldiazomethane (PDAM), a fluorescent labeling reagent for determination of carboxilic acids, using High Performance Liquid Chromatography with fluorescence on-line detection . This procedure was developed in order to have a quantitative method for DSP toxins analysis that would be useful for health public services and private shellfish industries. The features of this labeling reagent are compared against ADAM and used for quantitative analysis of DSP toxins in Chilean mussels and cultured dinoflagellates samples.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.2 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200007en10.4067/S0716-97602003000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DSP toxins Okadaic acid Dinophysistoxin-1 (DTX-1) Pyrenyldiazomethane (PDAM) Patagonian fjords Chile
spellingShingle	DSP toxins Okadaic acid Dinophysistoxin-1 (DTX-1) Pyrenyldiazomethane (PDAM) Patagonian fjords Chile GARCÍA,CARLOS PEREIRA,PAULO VALLE,LUIS LAGOS,NÉSTOR Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
description	Diarrhetic shellfish poisoning (DSP) is a gastrointestinal disease caused by lipid soluble polyether toxins produced by dinoflagellates and accumulated in shellfish. Diarrhetic shellfish poisoning is a worldwide threat to public health and the shellfish industry. To date, only four lipid soluble polyethers have been known as diarrhetic shellfish toxins. Among them, Okadaic acid (OA), Dinophysistoxin 1 (DTX-1, 35-methyl OA), Dinophysistoxin 2 (DTX-2, OA isomers) and Dinophysistoxin 3 (DTX-3, 7-O-acyl-35-methyl OA), all of which have free carboxilic groups. To perform quantitative analysis of DSP toxins in shellfish samples is a requirement, because DSP toxins are endemic in the Chilean mollusks of the southern regions, and although human symptoms of DSP appear relatively mild in comparison with the Paralytic Shellfish Poisoning (PSP), the necessity of monitoring the chronic effects of continued uptake of low doses of DSP toxins more closely is imperative, since DSP toxins have been described as potent tumor promoters. This paper shows the synthesis pathway of a chromophore, 1-pyrenyldiazomethane (PDAM), a fluorescent labeling reagent for determination of carboxilic acids, using High Performance Liquid Chromatography with fluorescence on-line detection . This procedure was developed in order to have a quantitative method for DSP toxins analysis that would be useful for health public services and private shellfish industries. The features of this labeling reagent are compared against ADAM and used for quantitative analysis of DSP toxins in Chilean mussels and cultured dinoflagellates samples.
author	GARCÍA,CARLOS PEREIRA,PAULO VALLE,LUIS LAGOS,NÉSTOR
author_facet	GARCÍA,CARLOS PEREIRA,PAULO VALLE,LUIS LAGOS,NÉSTOR
author_sort	GARCÍA,CARLOS
title	Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
title_short	Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
title_full	Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
title_fullStr	Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
title_full_unstemmed	Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent
title_sort	quantitation of diarrhetic shellfish poisoning toxins in chilean mussel using pyrenyldiazomethane as fluorescent labeling reagent
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200007
work_keys_str_mv	AT garciacarlos quantitationofdiarrheticshellfishpoisoningtoxinsinchileanmusselusingpyrenyldiazomethaneasfluorescentlabelingreagent AT pereirapaulo quantitationofdiarrheticshellfishpoisoningtoxinsinchileanmusselusingpyrenyldiazomethaneasfluorescentlabelingreagent AT valleluis quantitationofdiarrheticshellfishpoisoningtoxinsinchileanmusselusingpyrenyldiazomethaneasfluorescentlabelingreagent AT lagosnestor quantitationofdiarrheticshellfishpoisoningtoxinsinchileanmusselusingpyrenyldiazomethaneasfluorescentlabelingreagent
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Quantitation of diarrhetic shellfish poisoning toxins in Chilean Mussel using pyrenyldiazomethane as fluorescent labeling reagent

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