Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have dem...

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Autores principales:	VERA,MARÍA INÉS, MOLINA,ALFREDO, PINTO,RODRIGO, REYES,MAURICIO, ÁLVAREZ,MARCO, KRAUSKOPF,ERWIN, QUEZADA,CLAUDIA, TORRES,JORGE, KRAUSKOPF,MANUEL
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Materias:	ribosomal gene rRNA rDNA fish carp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014
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spelling	oai:scielo:S0716-976020030002000142003-11-19Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpioVERA,MARÍA INÉSMOLINA,ALFREDOPINTO,RODRIGOREYES,MAURICIOÁLVAREZ,MARCOKRAUSKOPF,ERWINQUEZADA,CLAUDIATORRES,JORGEKRAUSKOPF,MANUEL ribosomal gene rRNA rDNA fish carp The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.2 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014en10.4067/S0716-97602003000200014
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ribosomal gene rRNA rDNA fish carp
spellingShingle	ribosomal gene rRNA rDNA fish carp VERA,MARÍA INÉS MOLINA,ALFREDO PINTO,RODRIGO REYES,MAURICIO ÁLVAREZ,MARCO KRAUSKOPF,ERWIN QUEZADA,CLAUDIA TORRES,JORGE KRAUSKOPF,MANUEL Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
description	The seasonal adaptation of the teleost Cyprinus carpio to the cyclical changes of its habitat demands physiological compensatory responses. The process involves profound nucleolar adjustments and remarkable changes in rRNA synthesis, which affects ribosomal biosynthesis. In this context, we have demonstrated that the synthesis of several proteins involved in ribosomal biogenesis as protein kinase CK2, ribosomal protein L41 and nucleolin, as well as U3 snoRNP, are differentially regulated in summer-acclimatized carp compared to the cold-season adapted fish. To understand the mechanisms involved in the seasonal regulation of rRNA gene transcription, we have been studying the carp rDNA cistron structure. Because the cis-elements that regulate the expression of the tandem organized ribosomal genes are located in the non-transcribed intergenic spacer (IGS), we analyzed the primary structure of the carp rDNA gene IGS. The gene organization is similar to that described from other vertebrate species, including numerous repetitive sequences, the transcription start site, and some potential cis-elements such as ribosomal enhancers, proximal terminator and transcriptional terminators. Ribosomal DNA is a remarkable case of gene duplication and has been used as a model to test the concerted evolution theory. We performed sequence comparison analyses of 18S rRNA coding sequences from carp with different species, data with which an unrooted phylogram was constructed.
author	VERA,MARÍA INÉS MOLINA,ALFREDO PINTO,RODRIGO REYES,MAURICIO ÁLVAREZ,MARCO KRAUSKOPF,ERWIN QUEZADA,CLAUDIA TORRES,JORGE KRAUSKOPF,MANUEL
author_facet	VERA,MARÍA INÉS MOLINA,ALFREDO PINTO,RODRIGO REYES,MAURICIO ÁLVAREZ,MARCO KRAUSKOPF,ERWIN QUEZADA,CLAUDIA TORRES,JORGE KRAUSKOPF,MANUEL
author_sort	VERA,MARÍA INÉS
title	Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
title_short	Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
title_full	Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
title_fullStr	Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
title_full_unstemmed	Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio
title_sort	genomic organization of the rdna cistron of the teleost fish cyprinus carpio
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000200014
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_version_	1718441354125639680

Genomic organization of the rDNA cistron of the teleost fish Cyprinus carpio

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