Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions

The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was ca...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	CIFUENTES,ANA S, GONZÁLEZ,MARIELA A, VARGAS,SILVIA, HOENEISEN,MARITZA, GONZÁLEZ,NELSON
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Materias:	astaxanthin carotenogenesis growth Haematococcus pluvialis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602003000300006
record_format	dspace
spelling	oai:scielo:S0716-976020030003000062004-08-17Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditionsCIFUENTES,ANA SGONZÁLEZ,MARIELA AVARGAS,SILVIAHOENEISEN,MARITZAGONZÁLEZ,NELSON astaxanthin carotenogenesis growth Haematococcus pluvialis The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 µmol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8 %), 2) N-deprivation and 3) high PFD (150 µmol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k=0.7 div day-1, 228-258 mg l-1and 2.0 x 10(5) - 2.5 x 10(5) cells ml-1 at both PFD, respectively). With increasing acetate concentration, a slight increment in growth occurred only at 85 µmol m-2 s-1. Light was the best inductive carotenogenic factor, and the highest carotenoid production (4.9 mg l-1, 25.0 pg cell-1) was obtained in cultures pre-grown in nitrate at low light. The NaCl caused an increase in carotenoid content per cell at increasing salt concentrations, but resulted in a high cell mortality and did not produce any increment in carotenoid content per volume compared to cultures grown at 150 µmol m-2 s-1. The highest carotenoid content per cell (22 pg) and astaxanthin content per dry weight (10.3 mg g-1) (1% w/w) were obtained at 85 µmol m-2 s-1 with 0.8% NaCl.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.3-4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006en10.4067/S0716-97602003000300006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	astaxanthin carotenogenesis growth Haematococcus pluvialis
spellingShingle	astaxanthin carotenogenesis growth Haematococcus pluvialis CIFUENTES,ANA S GONZÁLEZ,MARIELA A VARGAS,SILVIA HOENEISEN,MARITZA GONZÁLEZ,NELSON Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
description	The microalga Haematococcus pluvialis Flotow is one of the natural sources of astaxanthin, a pigment widely used in salmon feed. This study was made to discover optimal conditions for biomass and astaxanthin production in H. pluvialis from Steptoe, Nevada (USA), cultured in batch mode. Growth was carried out under autotrophic (with NaNO3, NH4Cl and urea) and mixotrophic conditions (with 4, 8, 12 mM sodium acetate) under two photon flux densities (PFD) (35 and 85 µmol m-2 s-1). The carotenogenesis was induced by 1) addition of NaCl (0.2 and 0.8 %), 2) N-deprivation and 3) high PFD (150 µmol m-2 s-1). Total carotenoids were estimated by spectrophotometry and total astaxanthin by HPLC. Ammonium chloride was the best N-source for growth (k=0.7 div day-1, 228-258 mg l-1and 2.0 x 10(5) - 2.5 x 10(5) cells ml-1 at both PFD, respectively). With increasing acetate concentration, a slight increment in growth occurred only at 85 µmol m-2 s-1. Light was the best inductive carotenogenic factor, and the highest carotenoid production (4.9 mg l-1, 25.0 pg cell-1) was obtained in cultures pre-grown in nitrate at low light. The NaCl caused an increase in carotenoid content per cell at increasing salt concentrations, but resulted in a high cell mortality and did not produce any increment in carotenoid content per volume compared to cultures grown at 150 µmol m-2 s-1. The highest carotenoid content per cell (22 pg) and astaxanthin content per dry weight (10.3 mg g-1) (1% w/w) were obtained at 85 µmol m-2 s-1 with 0.8% NaCl.
author	CIFUENTES,ANA S GONZÁLEZ,MARIELA A VARGAS,SILVIA HOENEISEN,MARITZA GONZÁLEZ,NELSON
author_facet	CIFUENTES,ANA S GONZÁLEZ,MARIELA A VARGAS,SILVIA HOENEISEN,MARITZA GONZÁLEZ,NELSON
author_sort	CIFUENTES,ANA S
title	Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
title_short	Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
title_full	Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
title_fullStr	Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
title_full_unstemmed	Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions
title_sort	optimization of biomass, total carotenoids and astaxanthin production in haematococcus pluvialis flotow strain steptoe (nevada, usa) under laboratory conditions
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300006
work_keys_str_mv	AT cifuentesanas optimizationofbiomasstotalcarotenoidsandastaxanthinproductioninhaematococcuspluvialisflotowstrainsteptoenevadausaunderlaboratoryconditions AT gonzalezmarielaa optimizationofbiomasstotalcarotenoidsandastaxanthinproductioninhaematococcuspluvialisflotowstrainsteptoenevadausaunderlaboratoryconditions AT vargassilvia optimizationofbiomasstotalcarotenoidsandastaxanthinproductioninhaematococcuspluvialisflotowstrainsteptoenevadausaunderlaboratoryconditions AT hoeneisenmaritza optimizationofbiomasstotalcarotenoidsandastaxanthinproductioninhaematococcuspluvialisflotowstrainsteptoenevadausaunderlaboratoryconditions AT gonzaleznelson optimizationofbiomasstotalcarotenoidsandastaxanthinproductioninhaematococcuspluvialisflotowstrainsteptoenevadausaunderlaboratoryconditions
_version_	1718441356246908928

Optimization of biomass, total carotenoids and astaxanthin production in Haematococcus pluvialis Flotow strain Steptoe (Nevada, USA) under laboratory conditions

Ejemplares similares