Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation

Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation

Liver microsomal cytochrome P4502E1-dependent p-nitrophenol (PNP) hydroxylation and expression of cytochrome P4502E1 were studied in rats subjected to g-hexachlorocyclohexane (HCCH) or L-3,3,,5-triiodothyronine (T3) administration as a possible mechanism contributing to superoxide radical (O2.-) gen...

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Autores principales: FERNÁNDEZ,VIRGINIA, MASSA,LAURA, QUIÑONES,LUIS, SIMON-GIAVAROTTI,KARIN A, GIAVAROTTI,LEANDRO, D'ALMEIDA,VÂNIA, AZZALIS,LIGIA A, JUNQUEIRA,VIRGINIA BC, VIDELA,LUIS A
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2003
Materias:
g-Hexachlorocyclohexane
L-3,3',5-Triiodothyronine
Superoxide radical
Cytochrome P4502E1
Rat liver
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300007
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spelling oai:scielo:S0716-976020030003000072004-08-17Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generationFERNÁNDEZ,VIRGINIAMASSA,LAURAQUIÑONES,LUISSIMON-GIAVAROTTI,KARIN AGIAVAROTTI,LEANDROD'ALMEIDA,VÂNIAAZZALIS,LIGIA AJUNQUEIRA,VIRGINIA BCVIDELA,LUIS A g-Hexachlorocyclohexane L-3,3',5-Triiodothyronine Superoxide radical Cytochrome P4502E1 Rat liver Liver microsomal cytochrome P4502E1-dependent p-nitrophenol (PNP) hydroxylation and expression of cytochrome P4502E1 were studied in rats subjected to g-hexachlorocyclohexane (HCCH) or L-3,3,,5-triiodothyronine (T3) administration as a possible mechanism contributing to superoxide radical (O2.-) generation. HCCH treatment (a single dose of 40 mg/kg body wt) produced a 43% increase in the content of total cytochrome P450, whereas T3 (daily doses of 0.1 mg/kg body wt for two consecutive days) led to a 37% decrease. NADPH-dependent O2.- generation was elevated by HCCH and T3, expressed as either per mg of protein or per nmol of cytochrome P450, with a 135% enhancement in the O2.- production/superoxide dismutase (SOD) activity ratios being observed in both conditions. This was partly due to depression of SOD activity. Concomitantly, the molecular activity of NADPH-cytochrome p450 reductase was enhanced by 90 and 69% by HCCH and T3, respectively. In these conditions, microsomal PNP hydroxylation showed increases of 58 and 45% in HCCH- and T3-treated rats over control values, respectively, with a parallel 31% (HCCH) and 41% (T3) enhancement in the content of cytochrome P4502E1 assessed by western immunoblotting. We conclude that HCCH and T3 enhance the expression and activity of cytochrome P4502E1 and that of NADPH-cytochrome P450 reductase in rat liver, regardless of the changes in total cytochrome P450 content, representing major contributory mechanisms to microsomal NADPH-dependent O2.- generationinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.3-4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300007en10.4067/S0716-97602003000300007
institution Scielo Chile
collection Scielo Chile
language English
topic g-Hexachlorocyclohexane
L-3,3',5-Triiodothyronine
Superoxide radical
Cytochrome P4502E1
Rat liver
spellingShingle g-Hexachlorocyclohexane
L-3,3',5-Triiodothyronine
Superoxide radical
Cytochrome P4502E1
Rat liver
FERNÁNDEZ,VIRGINIA
MASSA,LAURA
QUIÑONES,LUIS
SIMON-GIAVAROTTI,KARIN A
GIAVAROTTI,LEANDRO
D'ALMEIDA,VÂNIA
AZZALIS,LIGIA A
JUNQUEIRA,VIRGINIA BC
VIDELA,LUIS A
Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
description Liver microsomal cytochrome P4502E1-dependent p-nitrophenol (PNP) hydroxylation and expression of cytochrome P4502E1 were studied in rats subjected to g-hexachlorocyclohexane (HCCH) or L-3,3,,5-triiodothyronine (T3) administration as a possible mechanism contributing to superoxide radical (O2.-) generation. HCCH treatment (a single dose of 40 mg/kg body wt) produced a 43% increase in the content of total cytochrome P450, whereas T3 (daily doses of 0.1 mg/kg body wt for two consecutive days) led to a 37% decrease. NADPH-dependent O2.- generation was elevated by HCCH and T3, expressed as either per mg of protein or per nmol of cytochrome P450, with a 135% enhancement in the O2.- production/superoxide dismutase (SOD) activity ratios being observed in both conditions. This was partly due to depression of SOD activity. Concomitantly, the molecular activity of NADPH-cytochrome p450 reductase was enhanced by 90 and 69% by HCCH and T3, respectively. In these conditions, microsomal PNP hydroxylation showed increases of 58 and 45% in HCCH- and T3-treated rats over control values, respectively, with a parallel 31% (HCCH) and 41% (T3) enhancement in the content of cytochrome P4502E1 assessed by western immunoblotting. We conclude that HCCH and T3 enhance the expression and activity of cytochrome P4502E1 and that of NADPH-cytochrome P450 reductase in rat liver, regardless of the changes in total cytochrome P450 content, representing major contributory mechanisms to microsomal NADPH-dependent O2.- generation
author FERNÁNDEZ,VIRGINIA
MASSA,LAURA
QUIÑONES,LUIS
SIMON-GIAVAROTTI,KARIN A
GIAVAROTTI,LEANDRO
D'ALMEIDA,VÂNIA
AZZALIS,LIGIA A
JUNQUEIRA,VIRGINIA BC
VIDELA,LUIS A
author_facet FERNÁNDEZ,VIRGINIA
MASSA,LAURA
QUIÑONES,LUIS
SIMON-GIAVAROTTI,KARIN A
GIAVAROTTI,LEANDRO
D'ALMEIDA,VÂNIA
AZZALIS,LIGIA A
JUNQUEIRA,VIRGINIA BC
VIDELA,LUIS A
author_sort FERNÁNDEZ,VIRGINIA
title Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
title_short Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
title_full Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
title_fullStr Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
title_full_unstemmed Effects of g-hexachlorocyclohexane and L-3,3',5- triiodothyronine on rat liver cytochrome P4502E1- dependent activity and content in relation to microsomal superoxide radical generation
title_sort effects of g-hexachlorocyclohexane and l-3,3',5- triiodothyronine on rat liver cytochrome p4502e1- dependent activity and content in relation to microsomal superoxide radical generation
publisher Sociedad de Biología de Chile
publishDate 2003
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300007
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