p53-Independent checkpoint controls in a plant cell model

Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	PELAYO,HELVIA R, PINCHEIRA,JUANA, GIMÉNEZ-ABIÁN,JUAN F, CLARKE,DUNCAN J, DE LA TORRE,CONSUELO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Materias:	Checkpoint adaptation Hydroxyurea S and G2 checkpoints p53-independent checkpoints Allium cepa L. meristems
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300009
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602003000300009
record_format	dspace
spelling	oai:scielo:S0716-976020030003000092005-07-11p53-Independent checkpoint controls in a plant cell modelPELAYO,HELVIA RPINCHEIRA,JUANAGIMÉNEZ-ABIÁN,JUAN FCLARKE,DUNCAN JDE LA TORRE,CONSUELO Checkpoint adaptation Hydroxyurea S and G2 checkpoints p53-independent checkpoints Allium cepa L. meristems Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had unduly overriden the G2 checkpoint block, without completing DNA repair. The frequency of the checkpoint-adapted mitoses increased with the hydroxyurea concentration from 0.25 to 1.0 mM. However, from 1 mM hydroxyurea upwards, some of the cells lost their competence for checkpoint adaptation. Therefore, the dose of a genotoxic agent that still allows G2 checkpoint adaptation should always be applied in order to get rid of uncontrolled proliferating cells. This is specially suitable for cells lacking a functional p53 proteininfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.3-4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300009en10.4067/S0716-97602003000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Checkpoint adaptation Hydroxyurea S and G2 checkpoints p53-independent checkpoints Allium cepa L. meristems
spellingShingle	Checkpoint adaptation Hydroxyurea S and G2 checkpoints p53-independent checkpoints Allium cepa L. meristems PELAYO,HELVIA R PINCHEIRA,JUANA GIMÉNEZ-ABIÁN,JUAN F CLARKE,DUNCAN J DE LA TORRE,CONSUELO p53-Independent checkpoint controls in a plant cell model
description	Allium cepa L. meristems were used as a plant model to study the p53-independent control of S and G2 phases by checkpoint pathways, in eukaryotic cells. Checkpoint blocks were induced at early and mid S by hydroxyurea. After their spontaneous override, cells became accumulated in G2-prophase, giving rise later on to a delayed mitotic wave. Cell growth was maintained during the checkpoint blocks, as the delayed mitoses were larger in size than the control ones. Under continuous hydroxyurea treatment, the delayed mitotic was formed by two subpopulations: normal mitoses corresponding to cells having properly recovered from the checkpoint block, and abnormal ones resulting from checkpoint adaptation. These latter cells displayed broken chromatids as they had unduly overriden the G2 checkpoint block, without completing DNA repair. The frequency of the checkpoint-adapted mitoses increased with the hydroxyurea concentration from 0.25 to 1.0 mM. However, from 1 mM hydroxyurea upwards, some of the cells lost their competence for checkpoint adaptation. Therefore, the dose of a genotoxic agent that still allows G2 checkpoint adaptation should always be applied in order to get rid of uncontrolled proliferating cells. This is specially suitable for cells lacking a functional p53 protein
author	PELAYO,HELVIA R PINCHEIRA,JUANA GIMÉNEZ-ABIÁN,JUAN F CLARKE,DUNCAN J DE LA TORRE,CONSUELO
author_facet	PELAYO,HELVIA R PINCHEIRA,JUANA GIMÉNEZ-ABIÁN,JUAN F CLARKE,DUNCAN J DE LA TORRE,CONSUELO
author_sort	PELAYO,HELVIA R
title	p53-Independent checkpoint controls in a plant cell model
title_short	p53-Independent checkpoint controls in a plant cell model
title_full	p53-Independent checkpoint controls in a plant cell model
title_fullStr	p53-Independent checkpoint controls in a plant cell model
title_full_unstemmed	p53-Independent checkpoint controls in a plant cell model
title_sort	p53-independent checkpoint controls in a plant cell model
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300009
work_keys_str_mv	AT pelayohelviar p53independentcheckpointcontrolsinaplantcellmodel AT pincheirajuana p53independentcheckpointcontrolsinaplantcellmodel AT gimenezabianjuanf p53independentcheckpointcontrolsinaplantcellmodel AT clarkeduncanj p53independentcheckpointcontrolsinaplantcellmodel AT delatorreconsuelo p53independentcheckpointcontrolsinaplantcellmodel
_version_	1718441356942114816

p53-Independent checkpoint controls in a plant cell model

Ejemplares similares