The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings

In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts fr...

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Autores principales:	FANTA,NORA, ORTEGA,XIMENA, PÉREZ,LUZ M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Materias:	Alternaria alternata lemon hypersensitive response Chitinases Glucanases
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012
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spelling	oai:scielo:S0716-976020030003000122004-08-17The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlingsFANTA,NORAORTEGA,XIMENAPÉREZ,LUZ M Alternaria alternata lemon hypersensitive response Chitinases Glucanases In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the detection of a new Ch isoenzyme and of three new Glu. Also, some constitutive Ch and Glu increased their enzyme activity, and those Ch that increased their activy also showed a broadening of their substrate specificity. These changes were prevented by a-amanitin and cycloheximide, suggesting that the presence of new Ch and Glu isoenzymes was due to de novo synthesis of proteins. Results suggest that constitutive Ch and Glu could act as pre-formed defense molecules in Citrus limon preventing A. alternata germination, while those induced after fungal inoculation of lemon seedlings could act along with the former, to produce lysis of fungal mycelia, resulting in a more efficient control of A. alternata development..info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.3-4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012en10.4067/S0716-97602003000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Alternaria alternata lemon hypersensitive response Chitinases Glucanases
spellingShingle	Alternaria alternata lemon hypersensitive response Chitinases Glucanases FANTA,NORA ORTEGA,XIMENA PÉREZ,LUZ M The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
description	In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the detection of a new Ch isoenzyme and of three new Glu. Also, some constitutive Ch and Glu increased their enzyme activity, and those Ch that increased their activy also showed a broadening of their substrate specificity. These changes were prevented by a-amanitin and cycloheximide, suggesting that the presence of new Ch and Glu isoenzymes was due to de novo synthesis of proteins. Results suggest that constitutive Ch and Glu could act as pre-formed defense molecules in Citrus limon preventing A. alternata germination, while those induced after fungal inoculation of lemon seedlings could act along with the former, to produce lysis of fungal mycelia, resulting in a more efficient control of A. alternata development..
author	FANTA,NORA ORTEGA,XIMENA PÉREZ,LUZ M
author_facet	FANTA,NORA ORTEGA,XIMENA PÉREZ,LUZ M
author_sort	FANTA,NORA
title	The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
title_short	The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
title_full	The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
title_fullStr	The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
title_full_unstemmed	The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
title_sort	development of alternaria alternata is prevented by chitinases and ß-1,3- glucanases from citrus limon seedlings
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012
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The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings

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