Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis

The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	WILHELM,VIVIAN, HUARACÁN,BERNARDO, MARTÍNEZ,RODRIGO, ROSEMBLATT,MARIO, BURZIO,LUIS O, VALENZUELA,PABLO D.T
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2003
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602003000300013
record_format	dspace
spelling	oai:scielo:S0716-976020030003000132004-08-17Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonisWILHELM,VIVIANHUARACÁN,BERNARDOMARTÍNEZ,RODRIGOROSEMBLATT,MARIOBURZIO,LUIS OVALENZUELA,PABLO D.TThe genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum from salmon infected with P. salmonis. The possibility of formulating a vaccine containing these two proteins is discussedinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.36 n.3-4 20032003-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013en10.4067/S0716-97602003000300013
institution	Scielo Chile
collection	Scielo Chile
language	English
description	The genes encoding the heat shock proteins HSP10 and HSP16 of the salmon pathogen Piscirickettsia salmonis have been isolated and sequenced. The HSP10 coding sequence is located in an open reading frame of 291 base pairs encoding 96 aminoacids. The HSP16 coding region was isolated as a 471 base pair fragment encoding a protein of 156 aminoacids. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from other prokaryotic organisms. Both proteins were expressed in E. coli as fusion proteins with thioredoxin and purified by chromatography on Ni-column. A rabbit serum against P. salmonis total proteins reacts with the recombinant HSP10 and HSP16 proteins. Similar reactivity was determined by ELISA using serum from salmon infected with P. salmonis. The possibility of formulating a vaccine containing these two proteins is discussed
author	WILHELM,VIVIAN HUARACÁN,BERNARDO MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T
spellingShingle	WILHELM,VIVIAN HUARACÁN,BERNARDO MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
author_facet	WILHELM,VIVIAN HUARACÁN,BERNARDO MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T
author_sort	WILHELM,VIVIAN
title	Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
title_short	Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
title_full	Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
title_fullStr	Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
title_full_unstemmed	Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis
title_sort	cloning and expression of the coding regions of the heat shock proteins hsp10 and hsp16 from piscirickettsia salmonis
publisher	Sociedad de Biología de Chile
publishDate	2003
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300013
work_keys_str_mv	AT wilhelmvivian cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis AT huaracanbernardo cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis AT martinezrodrigo cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis AT rosemblattmario cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis AT burzioluiso cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis AT valenzuelapablodt cloningandexpressionofthecodingregionsoftheheatshockproteinshsp10andhsp16frompiscirickettsiasalmonis
_version_	1718441358151122944

Cloning and expression of the coding regions of the heat shock proteins HSP10 and HSP16 from Piscirickettsia salmonis

Ejemplares similares