Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes

A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our...

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Autores principales:	CARRILLO,JUAN C, OJEDA,VERÓNICA A, CAMPOS-DE QUIROZ,HUGO A, ORTEGA,FERNANDO M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	In vitro culture meristem culture red clover Trifolium pratense L
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100005
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spelling	oai:scielo:S0716-976020040001000052004-09-09Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposesCARRILLO,JUAN COJEDA,VERÓNICA ACAMPOS-DE QUIROZ,HUGO AORTEGA,FERNANDO M In vitro culture meristem culture red clover Trifolium pratense L A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contamination and a better development than crown meristems. The L2 medium showed better results than B5 and MS media for the cultivars and experimental lines studied. L2 medium supplemented with 0.003 mg/l of 4-amino-3,5,6-trichloropicolinic acid and 1.0 mg/l of 6-benzylaminopurine gave consistently better results and will be applied in our breeding program to propagate, maintain and eliminate viruses from elite red clover clones.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.1 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100005en10.4067/S0716-97602004000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	In vitro culture meristem culture red clover Trifolium pratense L
spellingShingle	In vitro culture meristem culture red clover Trifolium pratense L CARRILLO,JUAN C OJEDA,VERÓNICA A CAMPOS-DE QUIROZ,HUGO A ORTEGA,FERNANDO M Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
description	A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contamination and a better development than crown meristems. The L2 medium showed better results than B5 and MS media for the cultivars and experimental lines studied. L2 medium supplemented with 0.003 mg/l of 4-amino-3,5,6-trichloropicolinic acid and 1.0 mg/l of 6-benzylaminopurine gave consistently better results and will be applied in our breeding program to propagate, maintain and eliminate viruses from elite red clover clones.
author	CARRILLO,JUAN C OJEDA,VERÓNICA A CAMPOS-DE QUIROZ,HUGO A ORTEGA,FERNANDO M
author_facet	CARRILLO,JUAN C OJEDA,VERÓNICA A CAMPOS-DE QUIROZ,HUGO A ORTEGA,FERNANDO M
author_sort	CARRILLO,JUAN C
title	Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
title_short	Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
title_full	Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
title_fullStr	Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
title_full_unstemmed	Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
title_sort	optimization of a protocol for direct organogenesis of red clover (trifolium pratense l.) meristems for breeding purposes
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100005
work_keys_str_mv	AT carrillojuanc optimizationofaprotocolfordirectorganogenesisofredclovertrifoliumpratenselmeristemsforbreedingpurposes AT ojedaveronicaa optimizationofaprotocolfordirectorganogenesisofredclovertrifoliumpratenselmeristemsforbreedingpurposes AT camposdequirozhugoa optimizationofaprotocolfordirectorganogenesisofredclovertrifoliumpratenselmeristemsforbreedingpurposes AT ortegafernandom optimizationofaprotocolfordirectorganogenesisofredclovertrifoliumpratenselmeristemsforbreedingpurposes
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Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes

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