Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.

Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growt...

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Autores principales:	MAYA,JUAN DIEGO, RODRÍGUEZ,ANDRÉS, PINO,LAURA, PABÓN,ADRIANA, FERREIRA,JORGE, PAVANI,MARIO, REPETTO,YOLANDA, MORELLO,ANTONIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Trypanosoma cruzi metallothionein glutathione trypanothione benznidazole nifurtimox buthionine sulfoximine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007
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spelling	oai:scielo:S0716-976020040001000072004-09-09Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.MAYA,JUAN DIEGORODRÍGUEZ,ANDRÉSPINO,LAURAPABÓN,ADRIANAFERREIRA,JORGEPAVANI,MARIOREPETTO,YOLANDAMORELLO,ANTONIO Trypanosoma cruzi metallothionein glutathione trypanothione benznidazole nifurtimox buthionine sulfoximine Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein, depending on the culture phase. The addition of buthionine sulfoximine to the protozoal culture considerably reduced the concentration of trypanothione and had no effect upon the metallothionein concentration. The possible contribution of metallothionein-like proteins to drug resistance in T. cruzi is discussed.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.1 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007en10.4067/S0716-97602004000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Trypanosoma cruzi metallothionein glutathione trypanothione benznidazole nifurtimox buthionine sulfoximine
spellingShingle	Trypanosoma cruzi metallothionein glutathione trypanothione benznidazole nifurtimox buthionine sulfoximine MAYA,JUAN DIEGO RODRÍGUEZ,ANDRÉS PINO,LAURA PABÓN,ADRIANA FERREIRA,JORGE PAVANI,MARIO REPETTO,YOLANDA MORELLO,ANTONIO Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
description	Proteins rich in sulfhydryl groups, such as metallothionein, are present in several strains of the parasite Trypanosoma cruzi, the etiological agent of Chagas' disease. Metallothionein-like protein concentrations ranged from 5.1 to 13.2 pmol/mg protein depending on the parasite strain and growth phase. Nifurtimox and benznidazole, used in the treatment of Chagas' disease, decreased metallothionein activity by approximately 70%. T. cruzi metallothionein was induced by ZnCl2. Metallothionein from T. cruzi was partially purified and its monobromobimane derivative showed a molecular weight of approximately 10,000 Da by SDS-PAGE analysis. The concentration of trypanothione, the major glutathione conjugate in T. cruzi, ranged from 3.8 to 10.8 nmol/mg protein, depending on the culture phase. The addition of buthionine sulfoximine to the protozoal culture considerably reduced the concentration of trypanothione and had no effect upon the metallothionein concentration. The possible contribution of metallothionein-like proteins to drug resistance in T. cruzi is discussed.
author	MAYA,JUAN DIEGO RODRÍGUEZ,ANDRÉS PINO,LAURA PABÓN,ADRIANA FERREIRA,JORGE PAVANI,MARIO REPETTO,YOLANDA MORELLO,ANTONIO
author_facet	MAYA,JUAN DIEGO RODRÍGUEZ,ANDRÉS PINO,LAURA PABÓN,ADRIANA FERREIRA,JORGE PAVANI,MARIO REPETTO,YOLANDA MORELLO,ANTONIO
author_sort	MAYA,JUAN DIEGO
title	Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
title_short	Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
title_full	Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
title_fullStr	Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
title_full_unstemmed	Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.
title_sort	effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in trypanosoma cruzi.
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100007
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Effects of buthionine sulfoximine nifurtimox and benznidazole upon trypanothione and metallothionein proteins in Trypanosoma cruzi.

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