Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants

Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicase...

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Autores principales:	EHRENFELD,NICOLE, ROMANO,EDUARDO, SERRANO,CAROLINA, ARCE-JOHNSON,PATRICIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Replicase PLRV replicase mediated resistance RolA promoter transgenic potatoes
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100008
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spelling	oai:scielo:S0716-976020040001000082004-09-09Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plantsEHRENFELD,NICOLEROMANO,EDUARDOSERRANO,CAROLINAARCE-JOHNSON,PATRICIO Replicase PLRV replicase mediated resistance RolA promoter transgenic potatoes Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicases are being developed to provide protection for plants against viral diseases. The purpose of this study was to compare the protection afforded by the differential expression of PLRV replicase transgene in potato plants cv. Desirée. Plants were genetically modified to express the complete sense PLRV replicase gene. Two constructions were used, one containing the constitutive 35SCaMV promoter and the other the phloem-specific RolA promoter from Agrobacterium rhizogenes. Transgenic plants were infected with PLRV in vitro, using infested aphids. In plants in which 35SCaMV controlled the expression of the PLRV replicase gene, signs of infection were initially detected, although most plants later developed a recovery phenotype showing undetectable virus levels 40 days after infection. In turn, those plants with the RolA promoter displayed an initial resistance that was later overcome. Different molecular mechanisms are likely to participate in the response to PLRV infection of these two types of transgenic plants.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.1 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100008en10.4067/S0716-97602004000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Replicase PLRV replicase mediated resistance RolA promoter transgenic potatoes
spellingShingle	Replicase PLRV replicase mediated resistance RolA promoter transgenic potatoes EHRENFELD,NICOLE ROMANO,EDUARDO SERRANO,CAROLINA ARCE-JOHNSON,PATRICIO Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
description	Potato leafroll virus (PLRV) is a major menace for the potato production all over the world. PLRV is transmitted by aphids, and until now, the only strategy available to control this pest has been to use large amounts of insecticides. Transgenic approaches involving the expression of viral replicases are being developed to provide protection for plants against viral diseases. The purpose of this study was to compare the protection afforded by the differential expression of PLRV replicase transgene in potato plants cv. Desirée. Plants were genetically modified to express the complete sense PLRV replicase gene. Two constructions were used, one containing the constitutive 35SCaMV promoter and the other the phloem-specific RolA promoter from Agrobacterium rhizogenes. Transgenic plants were infected with PLRV in vitro, using infested aphids. In plants in which 35SCaMV controlled the expression of the PLRV replicase gene, signs of infection were initially detected, although most plants later developed a recovery phenotype showing undetectable virus levels 40 days after infection. In turn, those plants with the RolA promoter displayed an initial resistance that was later overcome. Different molecular mechanisms are likely to participate in the response to PLRV infection of these two types of transgenic plants.
author	EHRENFELD,NICOLE ROMANO,EDUARDO SERRANO,CAROLINA ARCE-JOHNSON,PATRICIO
author_facet	EHRENFELD,NICOLE ROMANO,EDUARDO SERRANO,CAROLINA ARCE-JOHNSON,PATRICIO
author_sort	EHRENFELD,NICOLE
title	Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
title_short	Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
title_full	Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
title_fullStr	Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
title_full_unstemmed	Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants
title_sort	replicase mediated resistance against potato leafroll virus in potato desirée plants
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100008
work_keys_str_mv	AT ehrenfeldnicole replicasemediatedresistanceagainstpotatoleafrollvirusinpotatodesireeplants AT romanoeduardo replicasemediatedresistanceagainstpotatoleafrollvirusinpotatodesireeplants AT serranocarolina replicasemediatedresistanceagainstpotatoleafrollvirusinpotatodesireeplants AT arcejohnsonpatricio replicasemediatedresistanceagainstpotatoleafrollvirusinpotatodesireeplants
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Replicase mediated resistance against Potato Leafroll Virus in potato Desirée plants

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