Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)

The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to corre...

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Autores principales:	LODATO,PATRICIA, ALCAÍNO,JENNIFER, BARAHONA,SALVADOR, RETAMALES,PATRICIO, JIMÉNEZ,ANTONIO, CIFUENTES,VÍCTOR
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009
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spelling	oai:scielo:S0716-976020040001000092004-09-09Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)LODATO,PATRICIAALCAÍNO,JENNIFERBARAHONA,SALVADORRETAMALES,PATRICIOJIMÉNEZ,ANTONIOCIFUENTES,VÍCTOR Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was not correlated with the transcript level of carotenogenic genes under the same experimental conditions. This result suggests that there is not a linear relationship between carotenogenic transcript levels and carotenoid biosynthesis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.1 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009en10.4067/S0716-97602004000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma
spellingShingle	Carotenoid biosynthesis overproducer strains Xanthophyllomyces dendrorhous Phaffia rhodozyma LODATO,PATRICIA ALCAÍNO,JENNIFER BARAHONA,SALVADOR RETAMALES,PATRICIO JIMÉNEZ,ANTONIO CIFUENTES,VÍCTOR Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
description	The expression, at the mRNA level, of carotenoid biosynthetic genes from Xanthophyllomyces dendrorhous was studied by RT-PCR. The experimental conditions for the RT-PCR assay were standardized to quantify the relative transcript levels of idi, crtE, crtYB and crtI genes. This work attempted to correlate astaxanthin production with the transcript levels of carotenogenic genes in a wild-type strain (UCD 67-385) and two overproducer deregulated strains (atxS1 and atxS2). At 3 day cultures, the wild-type strain contained higher transcript levels from the crtE and crtYB genes on minimal medium than on rich medium. Similarly, carotenoid production was higher on minimal medium than on rich medium. However, carotenoid production in the atxS1 and atxS2 strains was not correlated with the transcript level of carotenogenic genes under the same experimental conditions. This result suggests that there is not a linear relationship between carotenogenic transcript levels and carotenoid biosynthesis.
author	LODATO,PATRICIA ALCAÍNO,JENNIFER BARAHONA,SALVADOR RETAMALES,PATRICIO JIMÉNEZ,ANTONIO CIFUENTES,VÍCTOR
author_facet	LODATO,PATRICIA ALCAÍNO,JENNIFER BARAHONA,SALVADOR RETAMALES,PATRICIO JIMÉNEZ,ANTONIO CIFUENTES,VÍCTOR
author_sort	LODATO,PATRICIA
title	Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
title_short	Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
title_full	Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
title_fullStr	Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
title_full_unstemmed	Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)
title_sort	study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of xanthophyllomyces dendrorhous (ex.: phaffia rhodozyma)
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100009
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Study of the expression of carotenoid biosynthesis genes in wild-type and deregulated strains of Xanthophyllomyces dendrorhous (Ex.: Phaffia rhodozyma)

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