Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols

Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE)...

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Autores principales:	Naito,Yuji, Shimozawa,Makoto, Manabe,Hiroki, Kuroda,Masaaki, Tomatsuri,Naoya, Uchiyama,Kazuhiko, Takagi,Tomohisa, Yoshida,Norimasa, Yoshikawa,Toshikazu
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Adhesion molecule monocyte-endothelial cell interaction oxysterol red wine extract
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009
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spelling	oai:scielo:S0716-976020040002000092005-01-06Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by OxysterolsNaito,YujiShimozawa,MakotoManabe,HirokiKuroda,MasaakiTomatsuri,NaoyaUchiyama,KazuhikoTakagi,TomohisaYoshida,NorimasaYoshikawa,Toshikazu Adhesion molecule monocyte-endothelial cell interaction oxysterol red wine extract Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol. Incubation of HAEC with oxysterols (10 muM) increased expression of adhesion molecules in a time-dependent manner. Pretreatment of HAEC with RWE at final concentrations of 1, 10, and 100 ng/ml significantly inhibited the increase of surface protein expression and mRNA levels. Adherence of monocytes to oxysterol-stimulated HAEC was increased compared to that of unstimulated cells. Treatment of HAEC with RWE significantly inhibited adherence of monocytes. These results suggest that RWE works as an anti-atherogenic agent through the inhibition of endothelial-dependent adhesive interactions with monocytes induced by oxysterolsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.2 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009en10.4067/S0716-97602004000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Adhesion molecule monocyte-endothelial cell interaction oxysterol red wine extract
spellingShingle	Adhesion molecule monocyte-endothelial cell interaction oxysterol red wine extract Naito,Yuji Shimozawa,Makoto Manabe,Hiroki Kuroda,Masaaki Tomatsuri,Naoya Uchiyama,Kazuhiko Takagi,Tomohisa Yoshida,Norimasa Yoshikawa,Toshikazu Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
description	Red wine polyphenolic compounds have been demonstrated to possess antioxidant properties, and several studies have suggested that they might constitute a relevant dietary factor in the protection from coronary heart disease. The aim of the present study is to examine whether red wine extracts (RWE) can ameliorate oxysterol-induced endothelial response, and whether inhibition of adhesion molecule expression is involved in monocyte adhesion to endothelial cells. Surface expression and mRNA levels of adhesion molecules (intercellular adhesion molecule 1 and vascular cell adhesion molecule 1) were determined by ELISA and RT-PCR performed on human aortic endothelial cells (HAEC) monolayers stimulated with 7b-hydroxycholesterol or 25-hydroxycholesterol. Incubation of HAEC with oxysterols (10 muM) increased expression of adhesion molecules in a time-dependent manner. Pretreatment of HAEC with RWE at final concentrations of 1, 10, and 100 ng/ml significantly inhibited the increase of surface protein expression and mRNA levels. Adherence of monocytes to oxysterol-stimulated HAEC was increased compared to that of unstimulated cells. Treatment of HAEC with RWE significantly inhibited adherence of monocytes. These results suggest that RWE works as an anti-atherogenic agent through the inhibition of endothelial-dependent adhesive interactions with monocytes induced by oxysterols
author	Naito,Yuji Shimozawa,Makoto Manabe,Hiroki Kuroda,Masaaki Tomatsuri,Naoya Uchiyama,Kazuhiko Takagi,Tomohisa Yoshida,Norimasa Yoshikawa,Toshikazu
author_facet	Naito,Yuji Shimozawa,Makoto Manabe,Hiroki Kuroda,Masaaki Tomatsuri,Naoya Uchiyama,Kazuhiko Takagi,Tomohisa Yoshida,Norimasa Yoshikawa,Toshikazu
author_sort	Naito,Yuji
title	Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
title_short	Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
title_full	Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
title_fullStr	Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
title_full_unstemmed	Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols
title_sort	inhibitory effects of red wine extracts on endothelial-dependent adhesive interactions with monocytes induced by oxysterols
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000200009
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Inhibitory Effects of Red Wine Extracts on Endothelial-Dependent Adhesive Interactions with Monocytes Induced by Oxysterols

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