Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major

Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium...

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Autores principales:	LANZA,HENRIQUE, AFONSO-CARDOSO,SANDRA R, SILVA,ADRIANO G, NAPOLITANO,DANIELLE R, ESPÍNDOLA,FOUED S, PENA,JANETHE D.O, SOUZA,MARIA A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	calcium Leishmania major macrophage magnesium nitric oxide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004
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spelling	oai:scielo:S0716-976020040003000042005-03-14Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania majorLANZA,HENRIQUEAFONSO-CARDOSO,SANDRA RSILVA,ADRIANO GNAPOLITANO,DANIELLE RESPÍNDOLA,FOUED SPENA,JANETHE D.OSOUZA,MARIA A calcium Leishmania major macrophage magnesium nitric oxide Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogeninfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.3 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004en10.4067/S0716-97602004000300004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	calcium Leishmania major macrophage magnesium nitric oxide
spellingShingle	calcium Leishmania major macrophage magnesium nitric oxide LANZA,HENRIQUE AFONSO-CARDOSO,SANDRA R SILVA,ADRIANO G NAPOLITANO,DANIELLE R ESPÍNDOLA,FOUED S PENA,JANETHE D.O SOUZA,MARIA A Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
description	Amastigotes of Leishmania major have a great ability to evade destruction in host cells. This study investigated the activation in resident, inflammatory macrophages and J774 cells in vitro treated with lipopolysaccharide (LPS), soluble Leishmania antigen (SLA), calcium ionophore (CaI) and magnesium (Mg2+) alone or combined. An increase in nitric oxide (NO) production was observed in J774 or inflammatory macrophages treated with LPS alone or in combination with SLA and CaI. The same treatments did not affect the NO release by resident macrophages. There was no interference in uptake of L. major but CaI decreased intracellular proliferation of the parasite. This study demonstrated the importance of CaI in decreasing L. major proliferation inside murine macrophages while Mg2+ seemed to increase parasite proliferation. These finding may help to understand the events involved in host cells' clearance of this pathogen
author	LANZA,HENRIQUE AFONSO-CARDOSO,SANDRA R SILVA,ADRIANO G NAPOLITANO,DANIELLE R ESPÍNDOLA,FOUED S PENA,JANETHE D.O SOUZA,MARIA A
author_facet	LANZA,HENRIQUE AFONSO-CARDOSO,SANDRA R SILVA,ADRIANO G NAPOLITANO,DANIELLE R ESPÍNDOLA,FOUED S PENA,JANETHE D.O SOUZA,MARIA A
author_sort	LANZA,HENRIQUE
title	Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
title_short	Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
title_full	Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
title_fullStr	Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
title_full_unstemmed	Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major
title_sort	comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by leishmania major
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300004
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Comparative effect of ion calcium and magnesium in the activation and infection of the murine macrophage by Leishmania major

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