Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells

In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extrace...

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Autores principales:	VELARDE,VICTORIA, DE LA CERDA,PAULA M, DUARTE,CLAUDIA, ARANCIBIA,FRANCISCA, ABBOTT,EDUARDO, GONZÁLEZ,ALEJANDRO, MORENO,FRANCESCA, JAFFA,AYAD A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	bradykinin, antioxidants migration signal transduction reactive oxygen species
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300007
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spelling	oai:scielo:S0716-976020040003000072005-03-14Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cellsVELARDE,VICTORIADE LA CERDA,PAULA MDUARTE,CLAUDIAARANCIBIA,FRANCISCAABBOTT,EDUARDOGONZÁLEZ,ALEJANDROMORENO,FRANCESCAJAFFA,AYAD A bradykinin, antioxidants migration signal transduction reactive oxygen species In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extracellular matrix production via redox-sensitive signaling pathways. We investigated the role(s) of ROS in proliferation, migration and collagen synthesis induced by BK in VSMC derived from Sprague Dawley rat aorta. BK (10 nM) increased VSMC proliferation by 30 % (n=5); this proliferation was inhibited by the antioxidants N-acetylcysteine (20 mM) and a-lipoic acid (LA, 250 mM). In addition, BK induced an increase in cell migration and in collagen levels that were blocked by LA. ROS production induced by BK (n=10) was significantly inhibited by bisindolylmaleimide (4mM) and by PD98059 (40mM). These results suggest that: 1) ROS participate in the mechanism(s) used by bradykinin to induce cellular proliferation; 2) bradykinin induces ROS generation through a pathway that involves the kinases PKC and MEK; and 3) ROS participate in the pathways mediating cell migration and the production of collagen as a response to treatment with bradykinin. To our knowledge, this is the first report describing mechanisms to explain the participation of ROS in the cellular proliferation and extracellular matrix pathway regulated by BKinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.3 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300007en10.4067/S0716-97602004000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bradykinin, antioxidants migration signal transduction reactive oxygen species
spellingShingle	bradykinin, antioxidants migration signal transduction reactive oxygen species VELARDE,VICTORIA DE LA CERDA,PAULA M DUARTE,CLAUDIA ARANCIBIA,FRANCISCA ABBOTT,EDUARDO GONZÁLEZ,ALEJANDRO MORENO,FRANCESCA JAFFA,AYAD A Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
description	In addition to the induction of cell proliferation and migration, bradykinin (BK) can increase c-fos mRNA expression, activate ERK 1/2 and generate reactive oxygen species (ROS) in vascular smooth muscle cells (VSMC). It is not known, however, whether BK can induce cellular proliferation and extracellular matrix production via redox-sensitive signaling pathways. We investigated the role(s) of ROS in proliferation, migration and collagen synthesis induced by BK in VSMC derived from Sprague Dawley rat aorta. BK (10 nM) increased VSMC proliferation by 30 % (n=5); this proliferation was inhibited by the antioxidants N-acetylcysteine (20 mM) and a-lipoic acid (LA, 250 mM). In addition, BK induced an increase in cell migration and in collagen levels that were blocked by LA. ROS production induced by BK (n=10) was significantly inhibited by bisindolylmaleimide (4mM) and by PD98059 (40mM). These results suggest that: 1) ROS participate in the mechanism(s) used by bradykinin to induce cellular proliferation; 2) bradykinin induces ROS generation through a pathway that involves the kinases PKC and MEK; and 3) ROS participate in the pathways mediating cell migration and the production of collagen as a response to treatment with bradykinin. To our knowledge, this is the first report describing mechanisms to explain the participation of ROS in the cellular proliferation and extracellular matrix pathway regulated by BK
author	VELARDE,VICTORIA DE LA CERDA,PAULA M DUARTE,CLAUDIA ARANCIBIA,FRANCISCA ABBOTT,EDUARDO GONZÁLEZ,ALEJANDRO MORENO,FRANCESCA JAFFA,AYAD A
author_facet	VELARDE,VICTORIA DE LA CERDA,PAULA M DUARTE,CLAUDIA ARANCIBIA,FRANCISCA ABBOTT,EDUARDO GONZÁLEZ,ALEJANDRO MORENO,FRANCESCA JAFFA,AYAD A
author_sort	VELARDE,VICTORIA
title	Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
title_short	Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
title_full	Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
title_fullStr	Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
title_full_unstemmed	Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
title_sort	role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300007
work_keys_str_mv	AT velardevictoria roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT delacerdapaulam roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT duarteclaudia roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT arancibiafrancisca roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT abbotteduardo roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT gonzalezalejandro roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT morenofrancesca roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells AT jaffaayada roleofreactiveoxygenspeciesinbradykinininducedproliferationofvascularsmoothmusclecells
_version_	1718441371240497152

Role of reactive oxygen species in bradykinin-induced proliferation of vascular smooth muscle cells

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