The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis

The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytot...

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Autores principales:	FELMER,RICARDO N, CLARK,JOHN A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	genetic ablation NTR/CB1954 adipocytes differentiation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009
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spelling	oai:scielo:S0716-976020040003000092005-03-14The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosisFELMER,RICARDO NCLARK,JOHN A genetic ablation NTR/CB1954 adipocytes differentiation The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a good alternative approach for gene therapy studies and a new inducible approach to manipulating the number of cells in tissues of transgenic animals and the ability to study the recovery of the tissue from cell damage or lossinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.3 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009en10.4067/S0716-97602004000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic ablation NTR/CB1954 adipocytes differentiation
spellingShingle	genetic ablation NTR/CB1954 adipocytes differentiation FELMER,RICARDO N CLARK,JOHN A The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
description	The feasibility of ablating differentiated adipocytes and the mechanism of cell ablation with a suitable prodrug activating system is described. The system is based on the use of E. coli nitroreductase (NTR) enzyme that activates certain nitro compounds, such as the antitumor drug CB1954, into cytotoxic DNA interstrand cross-linking agents. Differentiated preadipocyte cells (3T3L1) transfected with an aP2 driven nitroreductase construct were efficiently killed after incubation with medium containing the prodrug CB1954, while untransfected cells were not affected. It was demonstrated that the mechanism of cell ablation is apoptosis and that the system has a bystander effect mediated by a toxic metabolite of the prodrug. The described system should provide a good alternative approach for gene therapy studies and a new inducible approach to manipulating the number of cells in tissues of transgenic animals and the ability to study the recovery of the tissue from cell damage or loss
author	FELMER,RICARDO N CLARK,JOHN A
author_facet	FELMER,RICARDO N CLARK,JOHN A
author_sort	FELMER,RICARDO N
title	The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
title_short	The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
title_full	The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
title_fullStr	The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
title_full_unstemmed	The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis
title_sort	gene suicide system ntr/cb1954 causes ablation of differentiated 3t3l1 adipocytes by apoptosis
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000300009
work_keys_str_mv	AT felmerricardon thegenesuicidesystemntrcb1954causesablationofdifferentiated3t3l1adipocytesbyapoptosis AT clarkjohna thegenesuicidesystemntrcb1954causesablationofdifferentiated3t3l1adipocytesbyapoptosis AT felmerricardon genesuicidesystemntrcb1954causesablationofdifferentiated3t3l1adipocytesbyapoptosis AT clarkjohna genesuicidesystemntrcb1954causesablationofdifferentiated3t3l1adipocytesbyapoptosis
_version_	1718441371696627712

The gene suicide system NTR/CB1954 causes ablation of differentiated 3T3L1 adipocytes by apoptosis

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