Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle

Molecular understanding of the mechanism of excitation-contraction (EC) coupling in skeletal muscle has been made possible by cultured myotube models lacking specific dihydropyridine receptor (DHPR) subunits and ryanodine receptor type 1 (RyR1) isoforms. Transient expression of missing cDNAs in muta...

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Autores principales:	CORONADO,ROBERTO, AHERN,CHRIS A, SHERIDAN,DAVID C, CHENG,WEIJUN, CARBONNEAU,LEAH, BHATTACHARYA,DIPANKAR
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	L-type Ca2+ channel Ca2+ transients confocal imaging cDNA expression b1 KO myotubes DHPR voltage sensor
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400010
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spelling	oai:scielo:S0716-976020040004000102005-06-02Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscleCORONADO,ROBERTOAHERN,CHRIS ASHERIDAN,DAVID CCHENG,WEIJUNCARBONNEAU,LEAHBHATTACHARYA,DIPANKAR L-type Ca2+ channel Ca2+ transients confocal imaging cDNA expression b1 KO myotubes DHPR voltage sensor Molecular understanding of the mechanism of excitation-contraction (EC) coupling in skeletal muscle has been made possible by cultured myotube models lacking specific dihydropyridine receptor (DHPR) subunits and ryanodine receptor type 1 (RyR1) isoforms. Transient expression of missing cDNAs in mutant myotubes leads to a rapid recovery, within days, of various Ca2+ current and EC coupling phenotypes. These myotube models have thus permitted structure-function analysis of EC coupling domains present in the DHPR controlling the opening of RyR1. The purpose of this brief review is to highlight advances made by this laboratory towards understanding the contribution of domains present in a1S and b1a subunits of the skeletal DHPR to EC coupling signaling. Our main contention is that domains of the a1S II-III loop are necessary but not sufficient to recapitulate skeletal-type EC coupling. Rather, the structural unit that controls the EC coupling signal appears to be the a1S/b1a pairinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400010en10.4067/S0716-97602004000400010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	L-type Ca2+ channel Ca2+ transients confocal imaging cDNA expression b1 KO myotubes DHPR voltage sensor
spellingShingle	L-type Ca2+ channel Ca2+ transients confocal imaging cDNA expression b1 KO myotubes DHPR voltage sensor CORONADO,ROBERTO AHERN,CHRIS A SHERIDAN,DAVID C CHENG,WEIJUN CARBONNEAU,LEAH BHATTACHARYA,DIPANKAR Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
description	Molecular understanding of the mechanism of excitation-contraction (EC) coupling in skeletal muscle has been made possible by cultured myotube models lacking specific dihydropyridine receptor (DHPR) subunits and ryanodine receptor type 1 (RyR1) isoforms. Transient expression of missing cDNAs in mutant myotubes leads to a rapid recovery, within days, of various Ca2+ current and EC coupling phenotypes. These myotube models have thus permitted structure-function analysis of EC coupling domains present in the DHPR controlling the opening of RyR1. The purpose of this brief review is to highlight advances made by this laboratory towards understanding the contribution of domains present in a1S and b1a subunits of the skeletal DHPR to EC coupling signaling. Our main contention is that domains of the a1S II-III loop are necessary but not sufficient to recapitulate skeletal-type EC coupling. Rather, the structural unit that controls the EC coupling signal appears to be the a1S/b1a pair
author	CORONADO,ROBERTO AHERN,CHRIS A SHERIDAN,DAVID C CHENG,WEIJUN CARBONNEAU,LEAH BHATTACHARYA,DIPANKAR
author_facet	CORONADO,ROBERTO AHERN,CHRIS A SHERIDAN,DAVID C CHENG,WEIJUN CARBONNEAU,LEAH BHATTACHARYA,DIPANKAR
author_sort	CORONADO,ROBERTO
title	Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
title_short	Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
title_full	Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
title_fullStr	Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
title_full_unstemmed	Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
title_sort	functional equivalence of dihydropyridine receptor a1s and b1a subunits in triggering excitation-contraction coupling in skeletal muscle
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400010
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Functional equivalence of dihydropyridine receptor a1S and b1a subunits in triggering excitation-contraction coupling in skeletal muscle

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