Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers

Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single...

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Autores principales:	SCHNEIDER,MARTIN F, RODNEY,GEORGE G
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Excitation-Contraction Coupling Ca2+ Sparks IperatoxinA DP4 Calmodulin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400016
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spelling	oai:scielo:S0716-976020040004000162005-06-02Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibersSCHNEIDER,MARTIN FRODNEY,GEORGE G Excitation-Contraction Coupling Ca2+ Sparks IperatoxinA DP4 Calmodulin Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single channels in artificial membranes, the study of Ca2+ sparks provides a means to understand the regulation of a small group of RyRs in the environment of a functionally intact triad and in the presence of endogenous regulatory proteins. To gain insight into the mechanisms that regulate the gating of RyRs we have utilized laser scanning confocal microscopy to measure Ca2+ sparks in permeabilized frog skeletal muscle fibers. This review summarizes our recent studies using both exogenous (ImperatoxinA and domain peptides) and endogenous (calmodulin) modulators of RyR to gain insight into the number of RyR Ca2+ release channels underlying a Ca2+ spark, how domain-domain interactions within RyR regulate the functional state of the channel as well as gating mechanisms of RyR in living muscle fibersinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400016en10.4067/S0716-97602004000400016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Excitation-Contraction Coupling Ca2+ Sparks IperatoxinA DP4 Calmodulin
spellingShingle	Excitation-Contraction Coupling Ca2+ Sparks IperatoxinA DP4 Calmodulin SCHNEIDER,MARTIN F RODNEY,GEORGE G Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
description	Local discrete elevations in myoplasmic Ca2+ (Ca2+ sparks) arise from the opening of a small group of RyRs. Summation of a large number of Ca2+ sparks gives rise to the whole cell Ca2+ transient necessary for muscle contraction. Unlike sarcoplasmic reticulum vesicle preparations and isolated single channels in artificial membranes, the study of Ca2+ sparks provides a means to understand the regulation of a small group of RyRs in the environment of a functionally intact triad and in the presence of endogenous regulatory proteins. To gain insight into the mechanisms that regulate the gating of RyRs we have utilized laser scanning confocal microscopy to measure Ca2+ sparks in permeabilized frog skeletal muscle fibers. This review summarizes our recent studies using both exogenous (ImperatoxinA and domain peptides) and endogenous (calmodulin) modulators of RyR to gain insight into the number of RyR Ca2+ release channels underlying a Ca2+ spark, how domain-domain interactions within RyR regulate the functional state of the channel as well as gating mechanisms of RyR in living muscle fibers
author	SCHNEIDER,MARTIN F RODNEY,GEORGE G
author_facet	SCHNEIDER,MARTIN F RODNEY,GEORGE G
author_sort	SCHNEIDER,MARTIN F
title	Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
title_short	Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
title_full	Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
title_fullStr	Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
title_full_unstemmed	Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers
title_sort	peptide and protein modulation of local ca2+ release events in permeabilized skeletal muscle fibers
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400016
work_keys_str_mv	AT schneidermartinf peptideandproteinmodulationoflocalca2releaseeventsinpermeabilizedskeletalmusclefibers AT rodneygeorgeg peptideandproteinmodulationoflocalca2releaseeventsinpermeabilizedskeletalmusclefibers
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Peptide and protein modulation of local Ca2+ release events in permeabilized skeletal muscle fibers

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