Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells

We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to ba...

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Autores principales:	JAIMOVICH,ENRIQUE, ESPINOSA,ALEJANDRA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	skeletal transcription factors insulin IGF-I testosterone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018
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spelling	oai:scielo:S0716-976020040004000182005-06-02Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cellsJAIMOVICH,ENRIQUEESPINOSA,ALEJANDRA skeletal transcription factors insulin IGF-I testosterone We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The different intracellular Ca2+ patterns produced by electrical stimulation, testosterone, IGF-I and insulin, may help to understand the role of intracellular calcium kinetics in the regulation of gene expression by various stimuli in skeletal muscle cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018en10.4067/S0716-97602004000400018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	skeletal transcription factors insulin IGF-I testosterone
spellingShingle	skeletal transcription factors insulin IGF-I testosterone JAIMOVICH,ENRIQUE ESPINOSA,ALEJANDRA Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
description	We studied the effect of IGF-I, insulin and testosterone on intracellular Ca2+ in cultured muscle cells. Insulin produced a fast (<1 s) and transient [Ca2+] increase lasting less than 10 s. IGF-I induced a transient [Ca2+] increase, reaching a fluorescence peak 6 s after stimulus, to return to basal values after 60 s. Testosterone induced delayed (35 s) and long lasting (100-200 s) signals, frequently associated with oscillations. IGF-I, testosterone and electrical stimulation-induced Ca2+ signals were shown to be dependent on IP3 production. All of these Ca2+ signals were blocked by inhibitors of the IP3 pathway. On the other hand, insulin-induced Ca2+ increase was dependent on ryanodine receptors and blocked by either nifedipine or ryanodine. The different intracellular Ca2+ patterns produced by electrical stimulation, testosterone, IGF-I and insulin, may help to understand the role of intracellular calcium kinetics in the regulation of gene expression by various stimuli in skeletal muscle cells.
author	JAIMOVICH,ENRIQUE ESPINOSA,ALEJANDRA
author_facet	JAIMOVICH,ENRIQUE ESPINOSA,ALEJANDRA
author_sort	JAIMOVICH,ENRIQUE
title	Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
title_short	Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
title_full	Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
title_fullStr	Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
title_full_unstemmed	Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
title_sort	possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400018
work_keys_str_mv	AT jaimovichenrique possiblelinkofdifferentslowcalciumsignalsgeneratedbymembranepotentialandhormonestodifferentialgeneexpressioninculturedmusclecells AT espinosaalejandra possiblelinkofdifferentslowcalciumsignalsgeneratedbymembranepotentialandhormonestodifferentialgeneexpressioninculturedmusclecells
_version_	1718441376921681920

Possible link of different slow calcium signals generated by membrane potential and hormones to differential gene expression in cultured muscle cells

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