Imaging single-channel calcium microdomains by total internal reflection microscopy

The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electro...

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Autores principales:	DEMURO,ANGELO, PARKER,IAN
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	single-channel recording calcium imaging TIRFM N-type Ca2+ channels
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025
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spelling	oai:scielo:S0716-976020040004000252005-06-02Imaging single-channel calcium microdomains by total internal reflection microscopyDEMURO,ANGELOPARKER,IAN single-channel recording calcium imaging TIRFM N-type Ca2+ channels The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025en10.4067/S0716-97602004000400025
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	single-channel recording calcium imaging TIRFM N-type Ca2+ channels
spellingShingle	single-channel recording calcium imaging TIRFM N-type Ca2+ channels DEMURO,ANGELO PARKER,IAN Imaging single-channel calcium microdomains by total internal reflection microscopy
description	The microdomains of Ca2+ in the cytosol around the mouth of open Ca2+ channels are the basic `building blocks' from which cellular Ca2+ signals are constructed. Moreover, the kinetics of local [Ca2+] closely reflect channel gating, so their measurement holds promise as an alternative to electrophysiological patch-clamp recording as a means to study single channel behavior. We have thus explored the development of optical techniques capable of imaging single-channel Ca2+ signals with good spatial and temporal resolution, and describe results obtained using total internal reflection fluorescence microscopy to monitor Ca2+ influx through single N-type channels expressed in Xenopus oocytes
author	DEMURO,ANGELO PARKER,IAN
author_facet	DEMURO,ANGELO PARKER,IAN
author_sort	DEMURO,ANGELO
title	Imaging single-channel calcium microdomains by total internal reflection microscopy
title_short	Imaging single-channel calcium microdomains by total internal reflection microscopy
title_full	Imaging single-channel calcium microdomains by total internal reflection microscopy
title_fullStr	Imaging single-channel calcium microdomains by total internal reflection microscopy
title_full_unstemmed	Imaging single-channel calcium microdomains by total internal reflection microscopy
title_sort	imaging single-channel calcium microdomains by total internal reflection microscopy
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000400025
work_keys_str_mv	AT demuroangelo imagingsinglechannelcalciummicrodomainsbytotalinternalreflectionmicroscopy AT parkerian imagingsinglechannelcalciummicrodomainsbytotalinternalreflectionmicroscopy
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Imaging single-channel calcium microdomains by total internal reflection microscopy

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