Genetic polymorphism of clinical and environmental strains of Pichia anomala

In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspon...

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Autores principales:	REYES,EUGENIO, BARAHONA,SALVADOR, FISCHMAN,OLGA, NIKLITSCHEK,MAURICIO, BAEZA,MARCELO, CIFUENTES,VÍCTOR
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Pichia anomala 5.8S rDNA RAPD internal transcribed spacer antifungal drugs
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004
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spelling	oai:scielo:S0716-976020040005000042005-06-01Genetic polymorphism of clinical and environmental strains of Pichia anomalaREYES,EUGENIOBARAHONA,SALVADORFISCHMAN,OLGANIKLITSCHEK,MAURICIOBAEZA,MARCELOCIFUENTES,VÍCTOR Pichia anomala 5.8S rDNA RAPD internal transcribed spacer antifungal drugs In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the ribosomal 5.8S DNA, the yeast samples were divided into four groups, where the strains within each group had the same sequence. Of the analyzed yeast isolates, 78% were identified as Pichia anomala. Using RAPD analysis with seven different Operon primers, polymorphism was observed within the four groups. Our study highlights the growing importance of P. anomala in fungemic episodes in premature neonates. Furthermore, the methodologies used provide a powerful tool to identify and determine differences in similar strains of this yeastinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 suppl.A 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004en10.4067/S0716-97602004000500004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pichia anomala 5.8S rDNA RAPD internal transcribed spacer antifungal drugs
spellingShingle	Pichia anomala 5.8S rDNA RAPD internal transcribed spacer antifungal drugs REYES,EUGENIO BARAHONA,SALVADOR FISCHMAN,OLGA NIKLITSCHEK,MAURICIO BAEZA,MARCELO CIFUENTES,VÍCTOR Genetic polymorphism of clinical and environmental strains of Pichia anomala
description	In this work 20 clinical and 3 environmental yeast isolates were characterized by classical morphological and physiological methods, as well as molecular methods based on PCR of the ITS1-5.8S rDNA-ITS2 region. The characteristic morphology and biochemical profiles observed in these samples correspond to those described for the Pichia genera, more specifically to P. anomala. The profiles of susceptibility to five antifungal drugs were determined by two broth dilution methods. The results obtained by both methods were comparable and showed that clinical isolates presented more resistance to azoles, amphotericin B, and 5-fluorocytosine, than environmental ones did. By amplification and sequencing of internal transcribed spacers (ITS1 and ITS2) and the ribosomal 5.8S DNA, the yeast samples were divided into four groups, where the strains within each group had the same sequence. Of the analyzed yeast isolates, 78% were identified as Pichia anomala. Using RAPD analysis with seven different Operon primers, polymorphism was observed within the four groups. Our study highlights the growing importance of P. anomala in fungemic episodes in premature neonates. Furthermore, the methodologies used provide a powerful tool to identify and determine differences in similar strains of this yeast
author	REYES,EUGENIO BARAHONA,SALVADOR FISCHMAN,OLGA NIKLITSCHEK,MAURICIO BAEZA,MARCELO CIFUENTES,VÍCTOR
author_facet	REYES,EUGENIO BARAHONA,SALVADOR FISCHMAN,OLGA NIKLITSCHEK,MAURICIO BAEZA,MARCELO CIFUENTES,VÍCTOR
author_sort	REYES,EUGENIO
title	Genetic polymorphism of clinical and environmental strains of Pichia anomala
title_short	Genetic polymorphism of clinical and environmental strains of Pichia anomala
title_full	Genetic polymorphism of clinical and environmental strains of Pichia anomala
title_fullStr	Genetic polymorphism of clinical and environmental strains of Pichia anomala
title_full_unstemmed	Genetic polymorphism of clinical and environmental strains of Pichia anomala
title_sort	genetic polymorphism of clinical and environmental strains of pichia anomala
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500004
work_keys_str_mv	AT reyeseugenio geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala AT barahonasalvador geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala AT fischmanolga geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala AT niklitschekmauricio geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala AT baezamarcelo geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala AT cifuentesvictor geneticpolymorphismofclinicalandenvironmentalstrainsofpichiaanomala
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Genetic polymorphism of clinical and environmental strains of Pichia anomala

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