Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis

We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular we...

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Autores principales:	WILHELM,VIVIAN, MORALES,CARMEN, MARTÍNEZ,RODRIGO, ROSEMBLATT,MARIO, BURZIO,LUIS O, VALENZUELA,PABLO D. T
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2004
Materias:	Piscirickettsia salmonis MltB TbpB recombinant antigens recombinant vaccines
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008
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spelling	oai:scielo:S0716-976020040005000082005-06-01Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonisWILHELM,VIVIANMORALES,CARMENMARTÍNEZ,RODRIGOROSEMBLATT,MARIOBURZIO,LUIS OVALENZUELA,PABLO D. T Piscirickettsia salmonis MltB TbpB recombinant antigens recombinant vaccines We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant proteins. A similar response was elicited by both proteins when injected intraperitoneally in Atlantic salmon. The present data indicates that these proteins are good candidates to be used in formulations to study the protective immunity of salmon to infection by P. salmonis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.37 n.4 suppl.A 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008en10.4067/S0716-97602004000500008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Piscirickettsia salmonis MltB TbpB recombinant antigens recombinant vaccines
spellingShingle	Piscirickettsia salmonis MltB TbpB recombinant antigens recombinant vaccines WILHELM,VIVIAN MORALES,CARMEN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D. T Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
description	We have isolated and sequenced the genes encoding the membrane bound transglycosylase B (MltB) and the transferring binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis. The results of the sequence revealed two open reading frames that encode proteins with calculated molecular weights of 38,830 and 85,140. The deduced aminoacid sequences of both proteins show a significant homology to the respective protein from phylogenetically related microorganisms. Partial sequences coding the amino and carboxyl regions of MltB and a sequence of 761 base pairs encoding the amino region of TbpB have been expressed in E. coli. The strong humoral response elicited by these proteins in mouse confirmed the immunogenic properties of the recombinant proteins. A similar response was elicited by both proteins when injected intraperitoneally in Atlantic salmon. The present data indicates that these proteins are good candidates to be used in formulations to study the protective immunity of salmon to infection by P. salmonis.
author	WILHELM,VIVIAN MORALES,CARMEN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D. T
author_facet	WILHELM,VIVIAN MORALES,CARMEN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D. T
author_sort	WILHELM,VIVIAN
title	Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
title_short	Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
title_full	Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
title_fullStr	Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
title_full_unstemmed	Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis
title_sort	isolation and expression of the genes coding for the membrane bound transglycosylase b (mltb) and the transferrin binding protein b (tbpb) of the salmon pathogen piscirickettsia salmonis
publisher	Sociedad de Biología de Chile
publishDate	2004
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000500008
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Isolation and expression of the genes coding for the membrane bound transglycosylase B (MltB) and the transferrin binding protein B (TbpB) of the salmon pathogen Piscirickettsia salmonis

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