Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The pr...

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Autores principales:	WILHELM,VIVIAN, SOZA,CRISTIÁN, MARTÍNEZ,RODRIGO, ROSEMBLATT,MARIO, BURZIO,LUIS O, VALENZUELA,PABLO D.T.
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2005
Materias:	Piscirickettsia salmonis Hsp60 Hsp70 groEL dnaK immune response
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009
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spelling	oai:scielo:S0716-976020050001000092005-07-06Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonisWILHELM,VIVIANSOZA,CRISTIÁNMARTÍNEZ,RODRIGOROSEMBLATT,MARIOBURZIO,LUIS OVALENZUELA,PABLO D.T. Piscirickettsia salmonis Hsp60 Hsp70 groEL dnaK immune response We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.1 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009en10.4067/S0716-97602005000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Piscirickettsia salmonis Hsp60 Hsp70 groEL dnaK immune response
spellingShingle	Piscirickettsia salmonis Hsp60 Hsp70 groEL dnaK immune response WILHELM,VIVIAN SOZA,CRISTIÁN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T. Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
description	We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective immunity of fish to infection by P. salmonis and their potential use in recombinants vaccines against this intracellular pathogen.
author	WILHELM,VIVIAN SOZA,CRISTIÁN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T.
author_facet	WILHELM,VIVIAN SOZA,CRISTIÁN MARTÍNEZ,RODRIGO ROSEMBLATT,MARIO BURZIO,LUIS O VALENZUELA,PABLO D.T.
author_sort	WILHELM,VIVIAN
title	Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
title_short	Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
title_full	Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
title_fullStr	Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
title_full_unstemmed	Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
title_sort	production and immune response of recombinant hsp60 and hsp70 from the salmon pathogen piscirickettsia salmonis
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009
work_keys_str_mv	AT wilhelmvivian productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis AT sozacristian productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis AT martinezrodrigo productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis AT rosemblattmario productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis AT burzioluiso productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis AT valenzuelapablodt productionandimmuneresponseofrecombinanthsp60andhsp70fromthesalmonpathogenpiscirickettsiasalmonis
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Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis

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