A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses

Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapi...

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Autores principales: MEISEL,LEE, FONSECA,BEATRIZ, GONZÁLEZ,SUSANA, BAEZA-YATES,RICARDO, CAMBIAZO,VERONICA, CAMPOS,REINALDO, GONZALEZ,MAURICIO, ORELLANA,ARIEL, RETAMALES,JULIO, SILVA,HERMAN
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2005
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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spelling oai:scielo:S0716-976020050001000102005-07-06A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics AnalysesMEISEL,LEEFONSECA,BEATRIZGONZÁLEZ,SUSANABAEZA-YATES,RICARDOCAMBIAZO,VERONICACAMPOS,REINALDOGONZALEZ,MAURICIOORELLANA,ARIELRETAMALES,JULIOSILVA,HERMAN cDNA library fruit fruit trees functional genomics peach polysaccharides Prunus persica RNA isolation Rosaceae RT-PCR Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional genomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.1 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010en10.4067/S0716-97602005000100010
institution Scielo Chile
collection Scielo Chile
language English
topic cDNA library
fruit
fruit trees
functional genomics
peach
polysaccharides
Prunus persica
RNA isolation
Rosaceae
RT-PCR
spellingShingle cDNA library
fruit
fruit trees
functional genomics
peach
polysaccharides
Prunus persica
RNA isolation
Rosaceae
RT-PCR
MEISEL,LEE
FONSECA,BEATRIZ
GONZÁLEZ,SUSANA
BAEZA-YATES,RICARDO
CAMBIAZO,VERONICA
CAMPOS,REINALDO
GONZALEZ,MAURICIO
ORELLANA,ARIEL
RETAMALES,JULIO
SILVA,HERMAN
A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
description Prunus persica has been proposed as a genomic model for deciduous trees and the Rosaceae family. Optimized protocols for RNA isolation are necessary to further advance studies in this model species such that functional genomics analyses may be performed. Here we present an optimized protocol to rapidly and efficiently purify high quality total RNA from peach fruits (Prunus persica). Isolating high-quality RNA from fruit tissue is often difficult due to large quantities of polysaccharides and polyphenolic compounds that accumulate in this tissue and co-purify with the RNA. Here we demonstrate that a modified version of the method used to isolate RNA from pine trees and the woody plant Cinnamomun tenuipilum is ideal for isolating high quality RNA from the fruits of Prunus persica. This RNA may be used for many functional genomic based experiments such as RT-PCR and the construction of large-insert cDNA libraries.
author MEISEL,LEE
FONSECA,BEATRIZ
GONZÁLEZ,SUSANA
BAEZA-YATES,RICARDO
CAMBIAZO,VERONICA
CAMPOS,REINALDO
GONZALEZ,MAURICIO
ORELLANA,ARIEL
RETAMALES,JULIO
SILVA,HERMAN
author_facet MEISEL,LEE
FONSECA,BEATRIZ
GONZÁLEZ,SUSANA
BAEZA-YATES,RICARDO
CAMBIAZO,VERONICA
CAMPOS,REINALDO
GONZALEZ,MAURICIO
ORELLANA,ARIEL
RETAMALES,JULIO
SILVA,HERMAN
author_sort MEISEL,LEE
title A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
title_short A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
title_full A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
title_fullStr A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
title_full_unstemmed A Rapid and Efficient Method for Purifying High Quality Total RNA from Peaches (Prunus persica) for Functional Genomics Analyses
title_sort rapid and efficient method for purifying high quality total rna from peaches (prunus persica) for functional genomics analyses
publisher Sociedad de Biología de Chile
publishDate 2005
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100010
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