IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein

IP3 increase and de novo synthesis of scoparone are produced in the hypersensitive response (HR) of lemon seedlings against the fungus Alternaria alternata. To elucidate whether a G-protein and/or a protein tyrosine kinase (PTK) are involved in signal transduction leading to the production of such a...

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Autores principales:	ORTEGA,XIMENA, VELÁSQUEZ,JUAN CARLOS, PÉREZ,LUZ M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2005
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100011
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spelling	oai:scielo:S0716-976020050001000112005-07-06IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-proteinORTEGA,XIMENAVELÁSQUEZ,JUAN CARLOSPÉREZ,LUZ MIP3 increase and de novo synthesis of scoparone are produced in the hypersensitive response (HR) of lemon seedlings against the fungus Alternaria alternata. To elucidate whether a G-protein and/or a protein tyrosine kinase (PTK) are involved in signal transduction leading to the production of such a defensive response, we studied the HR in this plant system after treatment with G-protein activators alone and PTK inhibitors in the presence of fungal conidia. No changes in the level of IP3 were detected in response to the treatment with the G-protein activators cholera toxin or mastoparan, although the HR was observed in response to these compounds as determined by the scoparone synthesis. On the contrary, the PTK inhibitors lavendustin A and 2,5-dihidroxy methyl cinnamate (DHMC) not only prevented the IP3 changes observed in response to the fungal inoculation of lemon seedlings but also blocked the development of the HR. These results suggest that the IP3 changes observed in response to A. alternata require a PTK activity and are the result of a G-protein independent Phospholipase C activity, even though the activation of a G-protein can also lead to the development of a HR. Therefore, it appears that more than one signaling pathway may be activated for the development of HR in lemon seedlings: one involving a G-protein and the other involving a PTK-dependent PLC.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.1 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100011en10.4067/S0716-97602005000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
description	IP3 increase and de novo synthesis of scoparone are produced in the hypersensitive response (HR) of lemon seedlings against the fungus Alternaria alternata. To elucidate whether a G-protein and/or a protein tyrosine kinase (PTK) are involved in signal transduction leading to the production of such a defensive response, we studied the HR in this plant system after treatment with G-protein activators alone and PTK inhibitors in the presence of fungal conidia. No changes in the level of IP3 were detected in response to the treatment with the G-protein activators cholera toxin or mastoparan, although the HR was observed in response to these compounds as determined by the scoparone synthesis. On the contrary, the PTK inhibitors lavendustin A and 2,5-dihidroxy methyl cinnamate (DHMC) not only prevented the IP3 changes observed in response to the fungal inoculation of lemon seedlings but also blocked the development of the HR. These results suggest that the IP3 changes observed in response to A. alternata require a PTK activity and are the result of a G-protein independent Phospholipase C activity, even though the activation of a G-protein can also lead to the development of a HR. Therefore, it appears that more than one signaling pathway may be activated for the development of HR in lemon seedlings: one involving a G-protein and the other involving a PTK-dependent PLC.
author	ORTEGA,XIMENA VELÁSQUEZ,JUAN CARLOS PÉREZ,LUZ M
spellingShingle	ORTEGA,XIMENA VELÁSQUEZ,JUAN CARLOS PÉREZ,LUZ M IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
author_facet	ORTEGA,XIMENA VELÁSQUEZ,JUAN CARLOS PÉREZ,LUZ M
author_sort	ORTEGA,XIMENA
title	IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
title_short	IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
title_full	IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
title_fullStr	IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
title_full_unstemmed	IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein
title_sort	ip3 production in the hypersensitive response of lemon seedlings against alternaria alternata involves active protein tyrosine kinases but not a g-protein
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100011
work_keys_str_mv	AT ortegaximena ip3productioninthehypersensitiveresponseoflemonseedlingsagainstalternariaalternatainvolvesactiveproteintyrosinekinasesbutnotagprotein AT velasquezjuancarlos ip3productioninthehypersensitiveresponseoflemonseedlingsagainstalternariaalternatainvolvesactiveproteintyrosinekinasesbutnotagprotein AT perezluzm ip3productioninthehypersensitiveresponseoflemonseedlingsagainstalternariaalternatainvolvesactiveproteintyrosinekinasesbutnotagprotein
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IP3 production in the hypersensitive response of lemon seedlings against Alternaria alternata involves active protein tyrosine kinases but not a G-protein

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