Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria

An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC...

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Autores principales:	Rabelo-Gonçalves,Elizabeth M A, Nishimura,Nancy F, Murilo R Zeitune,José
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2005
Materias:	BALB/c mouse experimental model Helicobacter pylori
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012
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spelling	oai:scielo:S0716-976020050001000122005-07-06Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteriaRabelo-Gonçalves,Elizabeth M ANishimura,Nancy FMurilo R Zeitune,José BALB/c mouse experimental model Helicobacter pylori An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori induced lymphomonocytic infiltration that was present from days 7 to 60 in group GA. In group GB, it was only present from days 14 to 35. In conclusion, our data suggested that freezing altered pathogenic properties of H. pylori and probably inhibited expression of bacterial antigens and consequently the establishment and maintenance of infection. Although the animals developed mild duodenitis and gastritis, the BALB/c mouse is not susceptible to developing peptic ulcers during H. pylori infectioninfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.1 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012en10.4067/S0716-97602005000100012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	BALB/c mouse experimental model Helicobacter pylori
spellingShingle	BALB/c mouse experimental model Helicobacter pylori Rabelo-Gonçalves,Elizabeth M A Nishimura,Nancy F Murilo R Zeitune,José Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
description	An experimental model for H. pylori infection was established by intragastrically challenging BALB/c mice with 1 ml (10(8) CFU/ml) of suspension for two consecutive days. Animals were divided into three groups. GA: mice inoculated with fresh bacteria; GB: mice inoculated with frozen bacteria, and GC: mice inoculated with brucella broth (control group). Animals were killed at 7, 14, 21, 28, 35 and 60 days pi and fragments of stomach and duodenum were collected, paraffin embedded and stained by hematoxylin-eosin and Giemsa. The results showed that challenged mice exhibited mild duodenitis and gastritis. In group GA, infiltration in the duodenum was lymphoplasmacytic until day 35; in group GB, it was lymphomonocytic for 60 days pi. In the stomach, H. pylori induced lymphomonocytic infiltration that was present from days 7 to 60 in group GA. In group GB, it was only present from days 14 to 35. In conclusion, our data suggested that freezing altered pathogenic properties of H. pylori and probably inhibited expression of bacterial antigens and consequently the establishment and maintenance of infection. Although the animals developed mild duodenitis and gastritis, the BALB/c mouse is not susceptible to developing peptic ulcers during H. pylori infection
author	Rabelo-Gonçalves,Elizabeth M A Nishimura,Nancy F Murilo R Zeitune,José
author_facet	Rabelo-Gonçalves,Elizabeth M A Nishimura,Nancy F Murilo R Zeitune,José
author_sort	Rabelo-Gonçalves,Elizabeth M A
title	Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
title_short	Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
title_full	Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
title_fullStr	Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
title_full_unstemmed	Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria
title_sort	development of a balb/c mouse model of helicobacter pylori infection with fresh and frozen bacteria
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100012
work_keys_str_mv	AT rabelogoncalveselizabethma developmentofabalbcmousemodelofhelicobacterpyloriinfectionwithfreshandfrozenbacteria AT nishimuranancyf developmentofabalbcmousemodelofhelicobacterpyloriinfectionwithfreshandfrozenbacteria AT murilorzeitunejose developmentofabalbcmousemodelofhelicobacterpyloriinfectionwithfreshandfrozenbacteria
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Development of a BALB/c mouse model of Helicobacter pylori infection with fresh and frozen bacteria

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