Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia

This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sac...

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Autores principales:	TIIDUS,PETER M, DELLER,MIRADA, BOMBARDIER,ERIC, GÜL,MUSTAFA, LIU,X. LINDA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2005
Materias:	ischemia estrogen muscle damage skeletal muscle neutrophils
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011
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spelling	oai:scielo:S0716-976020050002000112007-01-04Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemiaTIIDUS,PETER MDELLER,MIRADABOMBARDIER,ERICGÜL,MUSTAFALIU,X. LINDA ischemia estrogen muscle damage skeletal muscle neutrophils This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant elevations of MPO, G6PD and <img border=0 width=8 height=15 id="_x0000_i1027" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU activities were observed at various time points post-ischemia. No systematic differences between genders were noted in any of the measures. Estrogen supplementation in both male and female animals failed to significantly attenuate post-ischemia increases in MPO, G6PD and <img border=0 width=8 height=15 id="_x0000_i1028" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU activities in any of the muscles studied and in some cases accentuated activities of some of these measures. Unlike previous findings following exercise in skeletal muscle, this study failed to demonstrate estrogen-induced attenuation of indices of neutrophil infiltration or damage in skeletal muscles of rats up to 72 hours following ischemia. This demonstrates that estrogen may not consistently attenuate neutrophil infiltration and that a number of variables including damage modality, tissue or estrogen level may influence this.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.2-3 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011en10.4067/S0716-97602005000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ischemia estrogen muscle damage skeletal muscle neutrophils
spellingShingle	ischemia estrogen muscle damage skeletal muscle neutrophils TIIDUS,PETER M DELLER,MIRADA BOMBARDIER,ERIC GÜL,MUSTAFA LIU,X. LINDA Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
description	This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant elevations of MPO, G6PD and <img border=0 width=8 height=15 id="_x0000_i1027" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU activities were observed at various time points post-ischemia. No systematic differences between genders were noted in any of the measures. Estrogen supplementation in both male and female animals failed to significantly attenuate post-ischemia increases in MPO, G6PD and <img border=0 width=8 height=15 id="_x0000_i1028" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU activities in any of the muscles studied and in some cases accentuated activities of some of these measures. Unlike previous findings following exercise in skeletal muscle, this study failed to demonstrate estrogen-induced attenuation of indices of neutrophil infiltration or damage in skeletal muscles of rats up to 72 hours following ischemia. This demonstrates that estrogen may not consistently attenuate neutrophil infiltration and that a number of variables including damage modality, tissue or estrogen level may influence this.
author	TIIDUS,PETER M DELLER,MIRADA BOMBARDIER,ERIC GÜL,MUSTAFA LIU,X. LINDA
author_facet	TIIDUS,PETER M DELLER,MIRADA BOMBARDIER,ERIC GÜL,MUSTAFA LIU,X. LINDA
author_sort	TIIDUS,PETER M
title	Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
title_short	Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
title_full	Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
title_fullStr	Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
title_full_unstemmed	Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
title_sort	estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011
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Estrogen supplementation failed to attenuate biochemical indices of neutrophil infiltration or damage in rat skeletal muscles following ischemia

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