A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans

The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted ups...

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Autores principales:	RIVAS,MARIELLA, SEEGER,MICHAEL, HOLMES,DAVID S, JEDLICKI,EUGENIA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2005
Materias:	A. ferrooxidans quorum-sensing LuxI and LuxR Lux box homoserine lactone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018
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spelling	oai:scielo:S0716-976020050002000182007-01-04A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidansRIVAS,MARIELLASEEGER,MICHAELHOLMES,DAVID SJEDLICKI,EUGENIA A. ferrooxidans quorum-sensing LuxI and LuxR Lux box homoserine lactone The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted upstream of afeI. A cloned copy of afeI, expressed in E. coli, encodes an enzyme that catalyzes the production of a diffusible compound identified by gas chromatography and mass spectrometry as an unsubstituted N-acyl homoserine lactone (AHL) of chain length C14. This AHL can be detected by a reporter strain of Sinorhizobium meliloti Rm41 suggesting that it is biologically active. The reporter strain also responds to extracts of the supernatant of A. ferrooxidans grown to early stationary phase in sulfur medium indicating that a diffusible AHL is produced by this microorganism. Semi-quantitative RT-PCR experiments indicate that afeI and afeR are expressed maximally in early stationary phase and are more expressed when A. ferrooxidans is grown in sulfur- rather than iron-containing medium. Given the predicted amino acid sequence and functional properties of AfeI and AfeR it is proposed that A. ferrooxidans has a quorum sensing system similar to the LuxI-LuxR paradigm.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.38 n.2-3 20052005-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018en10.4067/S0716-97602005000200018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	A. ferrooxidans quorum-sensing LuxI and LuxR Lux box homoserine lactone
spellingShingle	A. ferrooxidans quorum-sensing LuxI and LuxR Lux box homoserine lactone RIVAS,MARIELLA SEEGER,MICHAEL HOLMES,DAVID S JEDLICKI,EUGENIA A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
description	The genome of the acidophilic, proteobacterium Acidithiobacillus ferrooxidans, contains linked but divergently oriented genes, termed afeI and afeR, whose predicted protein products are significantly similar to the LuxI and LuxR families of proteins. A possible promoter and Lux box are predicted upstream of afeI. A cloned copy of afeI, expressed in E. coli, encodes an enzyme that catalyzes the production of a diffusible compound identified by gas chromatography and mass spectrometry as an unsubstituted N-acyl homoserine lactone (AHL) of chain length C14. This AHL can be detected by a reporter strain of Sinorhizobium meliloti Rm41 suggesting that it is biologically active. The reporter strain also responds to extracts of the supernatant of A. ferrooxidans grown to early stationary phase in sulfur medium indicating that a diffusible AHL is produced by this microorganism. Semi-quantitative RT-PCR experiments indicate that afeI and afeR are expressed maximally in early stationary phase and are more expressed when A. ferrooxidans is grown in sulfur- rather than iron-containing medium. Given the predicted amino acid sequence and functional properties of AfeI and AfeR it is proposed that A. ferrooxidans has a quorum sensing system similar to the LuxI-LuxR paradigm.
author	RIVAS,MARIELLA SEEGER,MICHAEL HOLMES,DAVID S JEDLICKI,EUGENIA
author_facet	RIVAS,MARIELLA SEEGER,MICHAEL HOLMES,DAVID S JEDLICKI,EUGENIA
author_sort	RIVAS,MARIELLA
title	A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
title_short	A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
title_full	A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
title_fullStr	A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
title_full_unstemmed	A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans
title_sort	lux-like quorum sensing system in the extreme acidophile acidithiobacillus ferrooxidans
publisher	Sociedad de Biología de Chile
publishDate	2005
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200018
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A Lux-like Quorum Sensing System in the Extreme Acidophile Acidithiobacillus ferrooxidans

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