Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum

Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duo...

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Autor principal:	FCOLLINS,JAMES
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	ATP7A copper transport iron-deficiency anemia microarray
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100004
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spelling	oai:scielo:S0716-976020060001000042006-06-30Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunumFCOLLINS,JAMES ATP7A copper transport iron-deficiency anemia microarray Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Results showed that several genes were similarly induced in both gut segments, including DMT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein, the Menkes copper ATPase (ATP7A), tripartitie motif protein 27, and the sodium-dependent vitamin C transporter. However, a subset of genes showed regulation in only one or the other gut segment. In duodenum only, gastrokine 1, trefoil factor 1 and claudin 2 were induced by iron-deficiency. Other genes previously identified were only regulated in the duodenum. Overall, these studies demonstrate similarities and distinct differences in the genetic response to iron deprivation in the duodenum versus jejunum and provide evidence that more distal gut segments also may play a role in increasing iron absorption in iron-deficiency anemia.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100004en10.4067/S0716-97602006000100004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	ATP7A copper transport iron-deficiency anemia microarray
spellingShingle	ATP7A copper transport iron-deficiency anemia microarray FCOLLINS,JAMES Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
description	Previous studies revealed novel genetic changes in the duodenal mucosa of iron-deprived rats during post-natal development. These observations are now extended to compare the genetic response to iron deficiency in the duodenum versus jejunum of 12-wk-old rats. cRNA samples were prepared from the duodenal and jejunal mucosa of three groups each of control and iron-deficient rats and hybridized with RAE 230A and 230B gene chips (Affymetrix). Stringent data reduction strategies were employed. Results showed that several genes were similarly induced in both gut segments, including DMT1, Dcytb, transferrin receptor 1, heme oxygenase 1, metallothionein, the Menkes copper ATPase (ATP7A), tripartitie motif protein 27, and the sodium-dependent vitamin C transporter. However, a subset of genes showed regulation in only one or the other gut segment. In duodenum only, gastrokine 1, trefoil factor 1 and claudin 2 were induced by iron-deficiency. Other genes previously identified were only regulated in the duodenum. Overall, these studies demonstrate similarities and distinct differences in the genetic response to iron deprivation in the duodenum versus jejunum and provide evidence that more distal gut segments also may play a role in increasing iron absorption in iron-deficiency anemia.
author	FCOLLINS,JAMES
author_facet	FCOLLINS,JAMES
author_sort	FCOLLINS,JAMES
title	Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
title_short	Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
title_full	Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
title_fullStr	Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
title_full_unstemmed	Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
title_sort	gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100004
work_keys_str_mv	AT fcollinsjames genechipanalysesrevealdifferentialgeneticresponsestoirondeficiencyinratduodenumandjejunum
_version_	1718441394307072000

Gene chip analyses reveal differential genetic responses to iron deficiency in rat duodenum and jejunum

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