DMT1: Which metals does it transport?

DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other m...

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Autores principales:	GARRICK,MICHAEL D, SINGLETON,STEVEN T, VARGAS,FARIDA, KUO,H-C, ZHAO,LIN, KNÖPFEL,MARTIN, DAVIDSON,TODD, COSTA,MAX, PARADKAR,PRASAD, ROTH,JEROME A, GARRICK,LAURA M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Metal ion transport DCT1 Nramp2 or SLC11A2 expression assay competition between metal ions
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009
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spelling	oai:scielo:S0716-976020060001000092006-06-30DMT1: Which metals does it transport?GARRICK,MICHAEL DSINGLETON,STEVEN TVARGAS,FARIDAKUO,H-CZHAO,LINKNÖPFEL,MARTINDAVIDSON,TODDCOSTA,MAXPARADKAR,PRASADROTH,JEROME AGARRICK,LAURA M Metal ion transport DCT1 Nramp2 or SLC11A2 expression assay competition between metal ions DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other metals evoked currents, but it is unclear if these metals are substrates for DMT1. This report summarizes progress on which metals DMT1 transports, focusing on results from the authors' labs. We recently cloned 1A/+IRE and 2/-IRE DMT1 isoforms to generate HEK293 cell lines that express them in a tetracycline-inducible fashion, then compared induced expression to uninduced expression and to endogenous DMT1 expression. Induced expression increases ~50x over endogenous expression and ~10x over uninduced levels. Fe2+, Mn2+, Ni2+ and Cu1+ or Cu2+ are transported. We also explored competition between metal ions using this system because incorporation essentially represents DMT1 transport and find this order for transport affinity: Mn>?Cd>?Fe>Pb~Co~Ni>Zn. The effects of decreased DMT1 also could be examined. The Belgrade rat has diminished DMT1 function and thus provides ways of testing. A series of DNA constructs that generate siRNAs specific for DMT1 or certain DMT1 isoforms yield another way to test DMT1-based transport.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009en10.4067/S0716-97602006000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Metal ion transport DCT1 Nramp2 or SLC11A2 expression assay competition between metal ions
spellingShingle	Metal ion transport DCT1 Nramp2 or SLC11A2 expression assay competition between metal ions GARRICK,MICHAEL D SINGLETON,STEVEN T VARGAS,FARIDA KUO,H-C ZHAO,LIN KNÖPFEL,MARTIN DAVIDSON,TODD COSTA,MAX PARADKAR,PRASAD ROTH,JEROME A GARRICK,LAURA M DMT1: Which metals does it transport?
description	DMT1 _ Divalent Metal (Ion) Transporter 1 or SLC11A2/DCT1/Nramp2 _ transports Fe2+ into the duodenum and out of the endosome during the transferrin cycle. DMT1 also is important in non-transferrin bound iron uptake. It plays similar roles in Mn2+ trafficking. Voltage clamping showed that six other metals evoked currents, but it is unclear if these metals are substrates for DMT1. This report summarizes progress on which metals DMT1 transports, focusing on results from the authors' labs. We recently cloned 1A/+IRE and 2/-IRE DMT1 isoforms to generate HEK293 cell lines that express them in a tetracycline-inducible fashion, then compared induced expression to uninduced expression and to endogenous DMT1 expression. Induced expression increases ~50x over endogenous expression and ~10x over uninduced levels. Fe2+, Mn2+, Ni2+ and Cu1+ or Cu2+ are transported. We also explored competition between metal ions using this system because incorporation essentially represents DMT1 transport and find this order for transport affinity: Mn>?Cd>?Fe>Pb~Co~Ni>Zn. The effects of decreased DMT1 also could be examined. The Belgrade rat has diminished DMT1 function and thus provides ways of testing. A series of DNA constructs that generate siRNAs specific for DMT1 or certain DMT1 isoforms yield another way to test DMT1-based transport.
author	GARRICK,MICHAEL D SINGLETON,STEVEN T VARGAS,FARIDA KUO,H-C ZHAO,LIN KNÖPFEL,MARTIN DAVIDSON,TODD COSTA,MAX PARADKAR,PRASAD ROTH,JEROME A GARRICK,LAURA M
author_facet	GARRICK,MICHAEL D SINGLETON,STEVEN T VARGAS,FARIDA KUO,H-C ZHAO,LIN KNÖPFEL,MARTIN DAVIDSON,TODD COSTA,MAX PARADKAR,PRASAD ROTH,JEROME A GARRICK,LAURA M
author_sort	GARRICK,MICHAEL D
title	DMT1: Which metals does it transport?
title_short	DMT1: Which metals does it transport?
title_full	DMT1: Which metals does it transport?
title_fullStr	DMT1: Which metals does it transport?
title_full_unstemmed	DMT1: Which metals does it transport?
title_sort	dmt1: which metals does it transport?
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100009
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DMT1: Which metals does it transport?

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