Antioxidant responses of cortex neurons to iron loading

Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in...

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Autores principales:	AGUIRRE,PABLA, MENA,NATALIA, TAPIA,VICTORIA, ROJAS,ALEJANDRO, ARREDONDO,MIGUEL, NÚÑEZ,MARCO TULIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	glutathione iron redox response cortical neurons cell death
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012
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spelling	oai:scielo:S0716-976020060001000122006-06-30Antioxidant responses of cortex neurons to iron loadingAGUIRRE,PABLAMENA,NATALIATAPIA,VICTORIAROJAS,ALEJANDROARREDONDO,MIGUELNÚÑEZ,MARCO TULIO glutathione iron redox response cortical neurons cell death Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidized glutathione levels in response to a protocol of iron accumulation. We found that massive death occurred after 2 days in culture with 10 mM Fe. Surviving cells developed an adaptative response that included increased synthesis of GSH and the maintenance of a glutathione-based reduction potential. These results highlight the fundamental role of glutathione homeostasis in the antioxidant response and provide novel insights into the adaptative mechanisms of neurons subjected to progressive iron loads.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012en10.4067/S0716-97602006000100012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	glutathione iron redox response cortical neurons cell death
spellingShingle	glutathione iron redox response cortical neurons cell death AGUIRRE,PABLA MENA,NATALIA TAPIA,VICTORIA ROJAS,ALEJANDRO ARREDONDO,MIGUEL NÚÑEZ,MARCO TULIO Antioxidant responses of cortex neurons to iron loading
description	Brain cells have a highly active oxidative metabolism, yet they contain only low to moderate superoxide dismutase and catalase activities. Thus, their antioxidant defenses rely mainly on cellular reduced glutathione levels. In this work, in cortical neurons we characterized viability and changes in reduced and oxidized glutathione levels in response to a protocol of iron accumulation. We found that massive death occurred after 2 days in culture with 10 mM Fe. Surviving cells developed an adaptative response that included increased synthesis of GSH and the maintenance of a glutathione-based reduction potential. These results highlight the fundamental role of glutathione homeostasis in the antioxidant response and provide novel insights into the adaptative mechanisms of neurons subjected to progressive iron loads.
author	AGUIRRE,PABLA MENA,NATALIA TAPIA,VICTORIA ROJAS,ALEJANDRO ARREDONDO,MIGUEL NÚÑEZ,MARCO TULIO
author_facet	AGUIRRE,PABLA MENA,NATALIA TAPIA,VICTORIA ROJAS,ALEJANDRO ARREDONDO,MIGUEL NÚÑEZ,MARCO TULIO
author_sort	AGUIRRE,PABLA
title	Antioxidant responses of cortex neurons to iron loading
title_short	Antioxidant responses of cortex neurons to iron loading
title_full	Antioxidant responses of cortex neurons to iron loading
title_fullStr	Antioxidant responses of cortex neurons to iron loading
title_full_unstemmed	Antioxidant responses of cortex neurons to iron loading
title_sort	antioxidant responses of cortex neurons to iron loading
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100012
work_keys_str_mv	AT aguirrepabla antioxidantresponsesofcortexneuronstoironloading AT menanatalia antioxidantresponsesofcortexneuronstoironloading AT tapiavictoria antioxidantresponsesofcortexneuronstoironloading AT rojasalejandro antioxidantresponsesofcortexneuronstoironloading AT arredondomiguel antioxidantresponsesofcortexneuronstoironloading AT nunezmarcotulio antioxidantresponsesofcortexneuronstoironloading
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Antioxidant responses of cortex neurons to iron loading

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