Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers
Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 5...
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Sociedad de Biología de Chile
2006
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oai:scielo:S0716-976020060001000162006-06-30Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayersLINDER,MARIA CMORIYA,MIZUEWHON,ANNEKASSA,AZEBGILLEY,CYNTHIA intestinal iron absorption Caco2 cells apotransferrin vesicular transport copper manganese Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed a single, saturable process for uptake of Fe(II) that was pH dependent and had a Km of 7 μM. An excess of Mn(II) and Cu(I) over Fe(II) of 200: 1 (μM: μM) in 1 mM ascorbate markedly inhibited Fe uptake. The kinetics were not competitive. Km increased and Vmax decreased. We conclude that vesicular transport, involving endo- and exocytosis at both ends of the enterocyte, is a fundamental aspect of intestinal iron absorption and that DMT1 may function as a transporter not just for divalent but also for monovalent metal ions.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016en10.4067/S0716-97602006000100016 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
intestinal iron absorption Caco2 cells apotransferrin vesicular transport copper manganese |
| spellingShingle |
intestinal iron absorption Caco2 cells apotransferrin vesicular transport copper manganese LINDER,MARIA C MORIYA,MIZUE WHON,ANNE KASSA,AZEB GILLEY,CYNTHIA Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| description |
Two aspects of the mechanisms by which iron is absorbed by the intestine were studied in the Caco2 cell model, using 59Fe(II)-ascorbate. Data showing the importance of vesicular processes and cycling of apotransferrin (apoTf) to uptake and overall transport of Caco2 cell monolayers (or basolateral 59Fe release) were obtained by comparing effects of: a) adding apoTf to the basal chamber; b) adding vesicular transport inhibitors; or c) cooling to 4ºC. These showed that apoTf may be involved in as much as half of Fe transfer across the basolateral membrane, and that vesicular processes may also play a role in non-apoTf-dependent Fe transport. Studies were initiated to examine potential interactions of other metal ions with Fe(II) via DMT1. Kinetic data showed a single, saturable process for uptake of Fe(II) that was pH dependent and had a Km of 7 μM. An excess of Mn(II) and Cu(I) over Fe(II) of 200: 1 (μM: μM) in 1 mM ascorbate markedly inhibited Fe uptake. The kinetics were not competitive. Km increased and Vmax decreased. We conclude that vesicular transport, involving endo- and exocytosis at both ends of the enterocyte, is a fundamental aspect of intestinal iron absorption and that DMT1 may function as a transporter not just for divalent but also for monovalent metal ions. |
| author |
LINDER,MARIA C MORIYA,MIZUE WHON,ANNE KASSA,AZEB GILLEY,CYNTHIA |
| author_facet |
LINDER,MARIA C MORIYA,MIZUE WHON,ANNE KASSA,AZEB GILLEY,CYNTHIA |
| author_sort |
LINDER,MARIA C |
| title |
Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| title_short |
Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| title_full |
Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| title_fullStr |
Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| title_full_unstemmed |
Vesicular transport of Fe and interaction with other metal ions in polarized Caco2 Cell monolayers |
| title_sort |
vesicular transport of fe and interaction with other metal ions in polarized caco2 cell monolayers |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2006 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100016 |
| work_keys_str_mv |
AT lindermariac vesiculartransportoffeandinteractionwithothermetalionsinpolarizedcaco2cellmonolayers AT moriyamizue vesiculartransportoffeandinteractionwithothermetalionsinpolarizedcaco2cellmonolayers AT whonanne vesiculartransportoffeandinteractionwithothermetalionsinpolarizedcaco2cellmonolayers AT kassaazeb vesiculartransportoffeandinteractionwithothermetalionsinpolarizedcaco2cellmonolayers AT gilleycynthia vesiculartransportoffeandinteractionwithothermetalionsinpolarizedcaco2cellmonolayers |
| _version_ |
1718441397256716288 |