Regulation of transepithelial transport of iron by hepcidin

Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spo...

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Autores principales:	MENA,NATALIA P, ESPARZA,ANDRÉS L, NÚÑEZ,MARCO T
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	hepcidin iron absorption DMT1 Caco-2 cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100022
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spelling	oai:scielo:S0716-976020060001000222006-06-30Regulation of transepithelial transport of iron by hepcidinMENA,NATALIA PESPARZA,ANDRÉS LNÚÑEZ,MARCO T hepcidin iron absorption DMT1 Caco-2 cells Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral iron overload. Lately, Hepc has been considered the "stores regulator," a putative factor that signals the iron content of the body to intestinal cells. In this work, we characterized the effect of Hepc produced by hepatoma cells on iron absorption by intestinal cells. To that end, human Hepc cDNA was cloned and overexpressed in HepG2 cells and conditioned media from Hepc-overexpressing cells was used to study the effects of Hepc on intestinal Caco-2 cells grown in bicameral inserts. The results indicate that Hepc released by HepG2 inhibited apical iron uptake by Caco-2 cells, probably by inhibiting the expression of the apical transporter DMT1. These results support a model in which Hepc released by the liver negatively regulates the expression of transporter DMT1 in the enterocyteinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100022en10.4067/S0716-97602006000100022
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	hepcidin iron absorption DMT1 Caco-2 cells
spellingShingle	hepcidin iron absorption DMT1 Caco-2 cells MENA,NATALIA P ESPARZA,ANDRÉS L NÚÑEZ,MARCO T Regulation of transepithelial transport of iron by hepcidin
description	Hepcidin (Hepc) is a 25 amino acid cationic peptide with broad antibacterial and antifungal actions. A likely role for Hepc in iron metabolism was suggested by the observation that mice having disruption of the gene encoding the transcription factor USF2 failed to produce Hepc mRNA and developed spontaneous visceral iron overload. Lately, Hepc has been considered the "stores regulator," a putative factor that signals the iron content of the body to intestinal cells. In this work, we characterized the effect of Hepc produced by hepatoma cells on iron absorption by intestinal cells. To that end, human Hepc cDNA was cloned and overexpressed in HepG2 cells and conditioned media from Hepc-overexpressing cells was used to study the effects of Hepc on intestinal Caco-2 cells grown in bicameral inserts. The results indicate that Hepc released by HepG2 inhibited apical iron uptake by Caco-2 cells, probably by inhibiting the expression of the apical transporter DMT1. These results support a model in which Hepc released by the liver negatively regulates the expression of transporter DMT1 in the enterocyte
author	MENA,NATALIA P ESPARZA,ANDRÉS L NÚÑEZ,MARCO T
author_facet	MENA,NATALIA P ESPARZA,ANDRÉS L NÚÑEZ,MARCO T
author_sort	MENA,NATALIA P
title	Regulation of transepithelial transport of iron by hepcidin
title_short	Regulation of transepithelial transport of iron by hepcidin
title_full	Regulation of transepithelial transport of iron by hepcidin
title_fullStr	Regulation of transepithelial transport of iron by hepcidin
title_full_unstemmed	Regulation of transepithelial transport of iron by hepcidin
title_sort	regulation of transepithelial transport of iron by hepcidin
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100022
work_keys_str_mv	AT menanataliap regulationoftransepithelialtransportofironbyhepcidin AT esparzaandresl regulationoftransepithelialtransportofironbyhepcidin AT nunezmarcot regulationoftransepithelialtransportofironbyhepcidin
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Regulation of transepithelial transport of iron by hepcidin

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