Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells

Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells

Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin...

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Autores principales: MENDIBURO,MARÍA JOSE, FLORES,SEBASTIÁN, PIZARRO,FERNANDO, ARREDONDO,MIGUEL
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2006
Materias:
iron
heme
heme oxygenase
Caco-2 cells
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100023
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spelling oai:scielo:S0716-976020060001000232006-06-30Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cellsMENDIBURO,MARÍA JOSEFLORES,SEBASTIÁNPIZARRO,FERNANDOARREDONDO,MIGUEL iron heme heme oxygenase Caco-2 cells Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed increased apical heme iron uptake and transepithelial transport when compared to control cells. These results suggested that heme oxygenase-1 mediates heme iron influx and efflux in intestinal cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100023en10.4067/S0716-97602006000100023
institution Scielo Chile
collection Scielo Chile
language English
topic iron
heme
heme oxygenase
Caco-2 cells
spellingShingle iron
heme
heme oxygenase
Caco-2 cells
MENDIBURO,MARÍA JOSE
FLORES,SEBASTIÁN
PIZARRO,FERNANDO
ARREDONDO,MIGUEL
Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
description Heme oxygenase-1 is a microsomal enzyme that, when induced by stress, protects the cells from oxidative injury. Heme oxygenase-1 participates in the cleavage of the heme ring producing biliverdin, CO and ferrous Fe. The released Fe becomes part of intracellular Fe pool and can be stored in ferritin or released by an iron exporter. The mechanism by which heme enters cells is not completely understood, although it had been suggested that it might be internalized by an endocytosis process. In this study, we expressed a full-length Heme oxygenase-1 cDNA in Caco-2 cells and measured intracellular iron content, heme-iron uptake and transport and immunolocalization of heme oxygenase-1 in these cells. We found that heme oxygenase-1 expressing cells showed increased apical heme iron uptake and transepithelial transport when compared to control cells. These results suggested that heme oxygenase-1 mediates heme iron influx and efflux in intestinal cells.
author MENDIBURO,MARÍA JOSE
FLORES,SEBASTIÁN
PIZARRO,FERNANDO
ARREDONDO,MIGUEL
author_facet MENDIBURO,MARÍA JOSE
FLORES,SEBASTIÁN
PIZARRO,FERNANDO
ARREDONDO,MIGUEL
author_sort MENDIBURO,MARÍA JOSE
title Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
title_short Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
title_full Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
title_fullStr Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
title_full_unstemmed Heme oxygenase 1 overexpression increases iron fluxes in Caco-2 cells
title_sort heme oxygenase 1 overexpression increases iron fluxes in caco-2 cells
publisher Sociedad de Biología de Chile
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100023
work_keys_str_mv AT mendiburomariajose hemeoxygenase1overexpressionincreasesironfluxesincaco2cells
AT floressebastian hemeoxygenase1overexpressionincreasesironfluxesincaco2cells
AT pizarrofernando hemeoxygenase1overexpressionincreasesironfluxesincaco2cells
AT arredondomiguel hemeoxygenase1overexpressionincreasesironfluxesincaco2cells
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