Characterization of mitochondrial iron uptake in HepG2 cells

There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mit...

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Autores principales:	MAZARIEGOS,DORA INÉS, CABANTCHIK,Z. IOAV, CASSELS,BRUCE K, NÚÑEZ,MARCO TULIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	mitochondria iron homeostasis RPA HepG2 cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024
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spelling	oai:scielo:S0716-976020060001000242006-06-30Characterization of mitochondrial iron uptake in HepG2 cellsMAZARIEGOS,DORA INÉSCABANTCHIK,Z. IOAVCASSELS,BRUCE KNÚÑEZ,MARCO TULIO mitochondria iron homeostasis RPA HepG2 cells There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.1 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024en10.4067/S0716-97602006000100024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mitochondria iron homeostasis RPA HepG2 cells
spellingShingle	mitochondria iron homeostasis RPA HepG2 cells MAZARIEGOS,DORA INÉS CABANTCHIK,Z. IOAV CASSELS,BRUCE K NÚÑEZ,MARCO TULIO Characterization of mitochondrial iron uptake in HepG2 cells
description	There is increasing evidence that accumulation of redox-active iron in mitochondria leads to oxidative damage and contributes to various neurodegenerative diseases, such as Friedreich's ataxia and Parkinson's disease. In this work, we examined the existence of regulatory mechanisms for mitochondrial iron uptake and storage. To that end, we used rhodamine B- [(1,10-phenanthrolin-5-yl)amino carbonyl ] benzyl ester, a new fluorescent iron-sensitive probe that is targeted specifically to the mitochondrion. We found that extracellular iron was incorporated readily into mitochondria in an apparently saturable process. Moreover, the rate of iron incorporation responded to the Fe status of the cell, an indication that the mitochondrion actively regulates its iron content.
author	MAZARIEGOS,DORA INÉS CABANTCHIK,Z. IOAV CASSELS,BRUCE K NÚÑEZ,MARCO TULIO
author_facet	MAZARIEGOS,DORA INÉS CABANTCHIK,Z. IOAV CASSELS,BRUCE K NÚÑEZ,MARCO TULIO
author_sort	MAZARIEGOS,DORA INÉS
title	Characterization of mitochondrial iron uptake in HepG2 cells
title_short	Characterization of mitochondrial iron uptake in HepG2 cells
title_full	Characterization of mitochondrial iron uptake in HepG2 cells
title_fullStr	Characterization of mitochondrial iron uptake in HepG2 cells
title_full_unstemmed	Characterization of mitochondrial iron uptake in HepG2 cells
title_sort	characterization of mitochondrial iron uptake in hepg2 cells
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000100024
work_keys_str_mv	AT mazariegosdoraines characterizationofmitochondrialironuptakeinhepg2cells AT cabantchikzioav characterizationofmitochondrialironuptakeinhepg2cells AT casselsbrucek characterizationofmitochondrialironuptakeinhepg2cells AT nunezmarcotulio characterizationofmitochondrialironuptakeinhepg2cells
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Characterization of mitochondrial iron uptake in HepG2 cells

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