Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant

Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both res...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	DÍAZ-GRIFFERO,FELIPE, ESPINOZA CANCINO,CARMEN, MEDINA ARÉVALO,CONSUELO, ARCE-JOHNSON,PATRICIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	movement protein crucifer-infecting tobamovirus viral complementation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200009
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602006000200009
record_format	dspace
spelling	oai:scielo:S0716-976020060002000092006-07-25Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutantDÍAZ-GRIFFERO,FELIPEESPINOZA CANCINO,CARMENMEDINA ARÉVALO,CONSUELOARCE-JOHNSON,PATRICIO movement protein crucifer-infecting tobamovirus viral complementation Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infectioninfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200009en10.4067/S0716-97602006000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	movement protein crucifer-infecting tobamovirus viral complementation
spellingShingle	movement protein crucifer-infecting tobamovirus viral complementation DÍAZ-GRIFFERO,FELIPE ESPINOZA CANCINO,CARMEN MEDINA ARÉVALO,CONSUELO ARCE-JOHNSON,PATRICIO Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
description	Tobamovirus movement proteins play a determinant role in the establishment of infections in plants, allowing the local movement of viral RNA genome through plasmodesmatas. We expressed the movement protein (MP) of the crucifer- and garlic-infecting Tobacco Mosaic Virus strain Cg (TMV-Cg) in both resistant Xanthi NN and sensitive Xanthi nn Nicotiana tabacum plants. MP-Cg function was assayed by inoculating transgenic plants with a trafficking-deficient mutant of TMV strain U1. Following infection, local necrotic lesions were developed in resistant transgenic plants, and a systemic infection was produced in sensitive tobaccos. Thus, movement function of the mutant virus was complemented in trans by MP-Cg expressed in transgenic plants, causing the same symptoms as wild-type strain. We demonstrated that the function of MP-U1 could be replaced efficiently by MP-Cg, even though these proteins share only 36% of identity. Similar hydrophobic patterns of MP-Cg and MP-U1 suggests structure and function conservations of both proteins. This work is an example of how two tobamoviruses differing in their host range help to understand viral movement mechanism during the infection
author	DÍAZ-GRIFFERO,FELIPE ESPINOZA CANCINO,CARMEN MEDINA ARÉVALO,CONSUELO ARCE-JOHNSON,PATRICIO
author_facet	DÍAZ-GRIFFERO,FELIPE ESPINOZA CANCINO,CARMEN MEDINA ARÉVALO,CONSUELO ARCE-JOHNSON,PATRICIO
author_sort	DÍAZ-GRIFFERO,FELIPE
title	Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
title_short	Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
title_full	Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
title_fullStr	Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
title_full_unstemmed	Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant
title_sort	expression of the crucifer-infecting tmv-cg movement protein in tobacco plants complements in trans a tmv-u1 trafficking-deficient mutant
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200009
work_keys_str_mv	AT diazgrifferofelipe expressionofthecruciferinfectingtmvcgmovementproteinintobaccoplantscomplementsintransatmvu1traffickingdeficientmutant AT espinozacancinocarmen expressionofthecruciferinfectingtmvcgmovementproteinintobaccoplantscomplementsintransatmvu1traffickingdeficientmutant AT medinaarevaloconsuelo expressionofthecruciferinfectingtmvcgmovementproteinintobaccoplantscomplementsintransatmvu1traffickingdeficientmutant AT arcejohnsonpatricio expressionofthecruciferinfectingtmvcgmovementproteinintobaccoplantscomplementsintransatmvu1traffickingdeficientmutant
_version_	1718441401574752256

Expression of the crucifer-infecting TMV-Cg movement protein in tobacco plants complements in trans a TMV-U1 trafficking-deficient mutant

Ejemplares similares