The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana

Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering...

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Autores principales:	URBINA,DANIELA C, SILVA,HERMAN, MEISEL,LEE A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Arabidopsis thaliana Ca2+-ATPases calcium gravitropism thapsigargin root
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011
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spelling	oai:scielo:S0716-976020060002000112006-07-25The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thalianaURBINA,DANIELA CSILVA,HERMANMEISEL,LEE A Arabidopsis thaliana Ca2+-ATPases calcium gravitropism thapsigargin root Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropisminfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011en10.4067/S0716-97602006000200011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Arabidopsis thaliana Ca2+-ATPases calcium gravitropism thapsigargin root
spellingShingle	Arabidopsis thaliana Ca2+-ATPases calcium gravitropism thapsigargin root URBINA,DANIELA C SILVA,HERMAN MEISEL,LEE A The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
description	Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism
author	URBINA,DANIELA C SILVA,HERMAN MEISEL,LEE A
author_facet	URBINA,DANIELA C SILVA,HERMAN MEISEL,LEE A
author_sort	URBINA,DANIELA C
title	The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
title_short	The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
title_full	The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
title_fullStr	The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
title_full_unstemmed	The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
title_sort	ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in arabidopsis thaliana
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011
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The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana

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