Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats

Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats

Nicotine exposure is a risk factor in several breathing disorders Nicotinic acetylcholine receptors (nAChRs) exist in the ventrolateral medulla, an important site for respiratory control. We examined the effects of nicotinic acetylcholine neurotransmission on central respiratory control by addition...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: HATORI,EIKI, SAKURABA,SHIGEKI, KASHIWAGI,MASANORI, KURIBAYASHI,JUNYA, TSUJITA,MIKI, HOSOKAWA,YUKI, TAKEDA,JUNZO, KUWANA,SHUN-ICHI
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2006
Materias:
brainstem-spinal cord
neonatal rat
inspiratory neuron
preinspiratory neuron
nicotinic acetylcholine receptor
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200014
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:scielo:S0716-97602006000200014
record_format dspace
spelling oai:scielo:S0716-976020060002000142006-07-25Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal ratsHATORI,EIKISAKURABA,SHIGEKIKASHIWAGI,MASANORIKURIBAYASHI,JUNYATSUJITA,MIKIHOSOKAWA,YUKITAKEDA,JUNZOKUWANA,SHUN-ICHI brainstem-spinal cord neonatal rat inspiratory neuron preinspiratory neuron nicotinic acetylcholine receptor Nicotine exposure is a risk factor in several breathing disorders Nicotinic acetylcholine receptors (nAChRs) exist in the ventrolateral medulla, an important site for respiratory control. We examined the effects of nicotinic acetylcholine neurotransmission on central respiratory control by addition of a nAChR agonist or one of various antagonists into superfusion medium in the isolated brainstem-spinal cord from neonatal rats. Ventral C4 neuronal activity was monitored as central respiratory output, and activities of respiratory neurons in the ventrolateral medulla were recorded in whole-cell configuration. RJR-2403 (0.1-10mM), alpha4beta2 nAChR agonist induced dose-dependent increases in respiratory frequency. Non-selective nAChR antagonist mecamylamine (0.1-100mM), alpha4beta2 antagonist dihydro-beta-erythroidine (0.1-100mM), alpha7 antagonist methyllycaconitine (0.1-100mM), and a-bungarotoxin (0.01-10mM) all induced dose-dependent reductions in C4 respiratory rate. We next examined effects of 20mM dihydro-beta-erythroidine and 20mM methyllycaconitine on respiratory neurons. Dihydro-beta-erythroidine induces hyperpolarization and decreases intraburst firing frequency of inspiratory and preinspiratory neurons. In contrast, methyllycaconitine has no effect on the membrane potential of inspiratory neurons, but does decrease their intraburst firing frequency while inducing hyperpolarization and decreasing intraburst firing frequency in preinspiratory neurons. These findings indicate that alpha4beta2 nAChR is involved in both inspiratory and preinspiratory neurons, whereas alpha7 nAChR functions only in preinspiratory neurons to modulate C4 respiratory rateinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200014en10.4067/S0716-97602006000200014
institution Scielo Chile
collection Scielo Chile
language English
topic brainstem-spinal cord
neonatal rat
inspiratory neuron
preinspiratory neuron
nicotinic acetylcholine receptor
spellingShingle brainstem-spinal cord
neonatal rat
inspiratory neuron
preinspiratory neuron
nicotinic acetylcholine receptor
HATORI,EIKI
SAKURABA,SHIGEKI
KASHIWAGI,MASANORI
KURIBAYASHI,JUNYA
TSUJITA,MIKI
HOSOKAWA,YUKI
TAKEDA,JUNZO
KUWANA,SHUN-ICHI
Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
description Nicotine exposure is a risk factor in several breathing disorders Nicotinic acetylcholine receptors (nAChRs) exist in the ventrolateral medulla, an important site for respiratory control. We examined the effects of nicotinic acetylcholine neurotransmission on central respiratory control by addition of a nAChR agonist or one of various antagonists into superfusion medium in the isolated brainstem-spinal cord from neonatal rats. Ventral C4 neuronal activity was monitored as central respiratory output, and activities of respiratory neurons in the ventrolateral medulla were recorded in whole-cell configuration. RJR-2403 (0.1-10mM), alpha4beta2 nAChR agonist induced dose-dependent increases in respiratory frequency. Non-selective nAChR antagonist mecamylamine (0.1-100mM), alpha4beta2 antagonist dihydro-beta-erythroidine (0.1-100mM), alpha7 antagonist methyllycaconitine (0.1-100mM), and a-bungarotoxin (0.01-10mM) all induced dose-dependent reductions in C4 respiratory rate. We next examined effects of 20mM dihydro-beta-erythroidine and 20mM methyllycaconitine on respiratory neurons. Dihydro-beta-erythroidine induces hyperpolarization and decreases intraburst firing frequency of inspiratory and preinspiratory neurons. In contrast, methyllycaconitine has no effect on the membrane potential of inspiratory neurons, but does decrease their intraburst firing frequency while inducing hyperpolarization and decreasing intraburst firing frequency in preinspiratory neurons. These findings indicate that alpha4beta2 nAChR is involved in both inspiratory and preinspiratory neurons, whereas alpha7 nAChR functions only in preinspiratory neurons to modulate C4 respiratory rate
author HATORI,EIKI
SAKURABA,SHIGEKI
KASHIWAGI,MASANORI
KURIBAYASHI,JUNYA
TSUJITA,MIKI
HOSOKAWA,YUKI
TAKEDA,JUNZO
KUWANA,SHUN-ICHI
author_facet HATORI,EIKI
SAKURABA,SHIGEKI
KASHIWAGI,MASANORI
KURIBAYASHI,JUNYA
TSUJITA,MIKI
HOSOKAWA,YUKI
TAKEDA,JUNZO
KUWANA,SHUN-ICHI
author_sort HATORI,EIKI
title Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
title_short Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
title_full Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
title_fullStr Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
title_full_unstemmed Association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
title_sort association of nicotinic acetylcholine receptors with central respiratory control in isolated brainstem-spinal cord preparation of neonatal rats
publisher Sociedad de Biología de Chile
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200014
work_keys_str_mv AT hatorieiki associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT sakurabashigeki associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT kashiwagimasanori associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT kuribayashijunya associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT tsujitamiki associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT hosokawayuki associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT takedajunzo associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
AT kuwanashunichi associationofnicotinicacetylcholinereceptorswithcentralrespiratorycontrolinisolatedbrainstemspinalcordpreparationofneonatalrats
_version_ 1718441402615988224

Ejemplares similares