The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells

Root growth, G2 length, and the frequency of aberrant mitoses and apoptotic nuclei were recorded after a single X-ray irradiation, ranging from 2.5 to 40 Gy, in Allium cepa L. root meristematic cells. After 72 h of recovery, root growth was reduced in a dose-dependent manner from 10 to 40 Gy, but no...

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Autores principales:	CARBALLO,JESÚS A, PINCHEIRA,JUANA, DE LA TORRE,CONSUELO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	apoptosis checkpoint adaptation G2 DNA damage checkpoint genome instability X-rays
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200015
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spelling	oai:scielo:S0716-976020060002000152006-07-25The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cellsCARBALLO,JESÚS APINCHEIRA,JUANADE LA TORRE,CONSUELO apoptosis checkpoint adaptation G2 DNA damage checkpoint genome instability X-rays Root growth, G2 length, and the frequency of aberrant mitoses and apoptotic nuclei were recorded after a single X-ray irradiation, ranging from 2.5 to 40 Gy, in Allium cepa L. root meristematic cells. After 72 h of recovery, root growth was reduced in a dose-dependent manner from 10 to 40 Gy, but not at 2.5 or 5 Gy doses. Flow cytometry plus TUNEL (TdT-mediated dUTP nick end labeling) showed that activation of apoptosis occurred only after 20 and 40 Gy of X-rays. Nevertheless, irrespective of the radiation dose, conventional flow cytometry showed that cells accumulated in G2 (4C DNA content). Simultaneously, the mitotic index fell, though a mitotic wave appeared later. Cell accumulation in G2 was transient and partially reversed by caffeine, thus it was checkpoint-dependent. Strikingly, the additional G2 time provided by this checkpoint was never long enough to complete DNA repair. Then, in all cases, some G2 cells with still-unrepaired DNA underwent checkpoint adaptation, i.e., they entered into the late mitotic wave with chromatid breaks. These cells and those produced by the breakage of chromosomal bridges in anaphase will reach the G1 of the next cell cycle unrepaired, ensuring the appearance of genome instabilityinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200015en10.4067/S0716-97602006000200015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	apoptosis checkpoint adaptation G2 DNA damage checkpoint genome instability X-rays
spellingShingle	apoptosis checkpoint adaptation G2 DNA damage checkpoint genome instability X-rays CARBALLO,JESÚS A PINCHEIRA,JUANA DE LA TORRE,CONSUELO The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
description	Root growth, G2 length, and the frequency of aberrant mitoses and apoptotic nuclei were recorded after a single X-ray irradiation, ranging from 2.5 to 40 Gy, in Allium cepa L. root meristematic cells. After 72 h of recovery, root growth was reduced in a dose-dependent manner from 10 to 40 Gy, but not at 2.5 or 5 Gy doses. Flow cytometry plus TUNEL (TdT-mediated dUTP nick end labeling) showed that activation of apoptosis occurred only after 20 and 40 Gy of X-rays. Nevertheless, irrespective of the radiation dose, conventional flow cytometry showed that cells accumulated in G2 (4C DNA content). Simultaneously, the mitotic index fell, though a mitotic wave appeared later. Cell accumulation in G2 was transient and partially reversed by caffeine, thus it was checkpoint-dependent. Strikingly, the additional G2 time provided by this checkpoint was never long enough to complete DNA repair. Then, in all cases, some G2 cells with still-unrepaired DNA underwent checkpoint adaptation, i.e., they entered into the late mitotic wave with chromatid breaks. These cells and those produced by the breakage of chromosomal bridges in anaphase will reach the G1 of the next cell cycle unrepaired, ensuring the appearance of genome instability
author	CARBALLO,JESÚS A PINCHEIRA,JUANA DE LA TORRE,CONSUELO
author_facet	CARBALLO,JESÚS A PINCHEIRA,JUANA DE LA TORRE,CONSUELO
author_sort	CARBALLO,JESÚS A
title	The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
title_short	The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
title_full	The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
title_fullStr	The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
title_full_unstemmed	The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells
title_sort	g2 checkpoint activated by dna damage does not prevent genome instability in plant cells
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200015
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The G2 checkpoint activated by DNA damage does not prevent genome instability in plant cells

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