Genomic organization of nucleolin gene in carp fish: Evidence for several genes

The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of...

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Autores principales:	QUEZADA,CLAUDIA, NAVARRO,CRISTINA, SAN MARTÍN,RODY, ÁLVAREZ,MARCO, MOLINA,ALFREDO, VERA,M. INÉS
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	nucleolin nucleolus U snoRNAs rRNA 18S carp fish
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017
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spelling	oai:scielo:S0716-976020060002000172006-07-25Genomic organization of nucleolin gene in carp fish: Evidence for several genesQUEZADA,CLAUDIANAVARRO,CRISTINASAN MARTÍN,RODYÁLVAREZ,MARCOMOLINA,ALFREDOVERA,M. INÉS nucleolin nucleolus U snoRNAs rRNA 18S carp fish The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory elements are similar to constitutive genes, i.e., a putative TATA box, three G/C boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene introns host three snoRNA codifying sequences: U23 from the H/ACA family and two C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary sequence with carp 18S rRNA. Additionally, we identified two cDNAs coding for nucleolin, confirming the existence of several nucleolin genes in carp. Amino acid-derived sequence from carp cDNAs differ from mammal protein because they span additional acidic domains at the amino end, whose functional significance remains unclear. We performed amino acid sequence comparison and phylogenetic analyses showing that the three isoforms of carp nucleolin, which we describe herein, cluster in two groups. cNUC1 probably diverges from cNUC2 and cNUC3 as result of ancestral fish-specific genome duplication, indeed C. carpio is a tetraploid fishinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017en10.4067/S0716-97602006000200017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	nucleolin nucleolus U snoRNAs rRNA 18S carp fish
spellingShingle	nucleolin nucleolus U snoRNAs rRNA 18S carp fish QUEZADA,CLAUDIA NAVARRO,CRISTINA SAN MARTÍN,RODY ÁLVAREZ,MARCO MOLINA,ALFREDO VERA,M. INÉS Genomic organization of nucleolin gene in carp fish: Evidence for several genes
description	The protein nucleolin, functionally involved in the main steps of ribosome biogenesis, is codified by a single copy gene in mammals. Here we report that at least three different genes codify for this protein in carp fish (Cyprinus carpio). This is the first description of the genomic organization of nucleolin in a teleost. The carp nucleolin gene includes 8.8 kb and contains 16 exons. Promoter cis regulatory elements are similar to constitutive genes, i.e., a putative TATA box, three G/C boxes, and three pyrimidine-rich boxes. As in other species, carp nucleolin gene introns host three snoRNA codifying sequences: U23 from the H/ACA family and two C/D box snoRNAs, U20 and U82. Both U20 and U82 span a complementary sequence with carp 18S rRNA. Additionally, we identified two cDNAs coding for nucleolin, confirming the existence of several nucleolin genes in carp. Amino acid-derived sequence from carp cDNAs differ from mammal protein because they span additional acidic domains at the amino end, whose functional significance remains unclear. We performed amino acid sequence comparison and phylogenetic analyses showing that the three isoforms of carp nucleolin, which we describe herein, cluster in two groups. cNUC1 probably diverges from cNUC2 and cNUC3 as result of ancestral fish-specific genome duplication, indeed C. carpio is a tetraploid fish
author	QUEZADA,CLAUDIA NAVARRO,CRISTINA SAN MARTÍN,RODY ÁLVAREZ,MARCO MOLINA,ALFREDO VERA,M. INÉS
author_facet	QUEZADA,CLAUDIA NAVARRO,CRISTINA SAN MARTÍN,RODY ÁLVAREZ,MARCO MOLINA,ALFREDO VERA,M. INÉS
author_sort	QUEZADA,CLAUDIA
title	Genomic organization of nucleolin gene in carp fish: Evidence for several genes
title_short	Genomic organization of nucleolin gene in carp fish: Evidence for several genes
title_full	Genomic organization of nucleolin gene in carp fish: Evidence for several genes
title_fullStr	Genomic organization of nucleolin gene in carp fish: Evidence for several genes
title_full_unstemmed	Genomic organization of nucleolin gene in carp fish: Evidence for several genes
title_sort	genomic organization of nucleolin gene in carp fish: evidence for several genes
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200017
work_keys_str_mv	AT quezadaclaudia genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes AT navarrocristina genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes AT sanmartinrody genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes AT alvarezmarco genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes AT molinaalfredo genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes AT veramines genomicorganizationofnucleolingeneincarpfishevidenceforseveralgenes
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Genomic organization of nucleolin gene in carp fish: Evidence for several genes

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