Generation and analysis of expressed sequence tags from Botrytis cinerea

Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags th...

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Autores principales:	SILVA,EVELYN, VALDÉS,JORGE, HOLMES,DAVID, SHMARYAHU,AMIR, VALENZUELA,PABLO D T
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Botrytis cinerea cDNA sequencing fungal pathogenicity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018
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spelling	oai:scielo:S0716-976020060002000182006-07-25Generation and analysis of expressed sequence tags from Botrytis cinereaSILVA,EVELYNVALDÉS,JORGEHOLMES,DAVIDSHMARYAHU,AMIRVALENZUELA,PABLO D T Botrytis cinerea cDNA sequencing fungal pathogenicity Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogeninfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.2 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018en10.4067/S0716-97602006000200018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Botrytis cinerea cDNA sequencing fungal pathogenicity
spellingShingle	Botrytis cinerea cDNA sequencing fungal pathogenicity SILVA,EVELYN VALDÉS,JORGE HOLMES,DAVID SHMARYAHU,AMIR VALENZUELA,PABLO D T Generation and analysis of expressed sequence tags from Botrytis cinerea
description	Botrytis cinerea is a filamentous plant pathogen of a wide range of plant species, and its infection may cause enormous damage both during plant growth and in the post-harvest phase. We have constructed a cDNA library from an isolate of B. cinerea and have sequenced 11,482 expressed sequence tags that were assembled into 1,003 contigs sequences and 3,032 singletons. Approximately 81% of the unigenes showed significant similarity to genes coding for proteins with known functions: more than 50% of the sequences code for genes involved in cellular metabolism, 12% for transport of metabolites, and approximately 10% for cellular organization. Other functional categories include responses to biotic and abiotic stimuli, cell communication, cell homeostasis, and cell development. We carried out pair-wise comparisons with fungal databases to determine the B. cinerea unisequence set with relevant similarity to genes in other fungal pathogenic counterparts. Among the 4,035 non-redundant B. cinerea unigenes, 1,338 (23%) have significant homology with Fusarium verticillioides unigenes. Similar values were obtained for Saccharomyces cerevisiae and Aspergillus nidulans (22% and 24%, respectively). The lower percentages of homology were with Magnaporthe grisae and Neurospora crassa (13% and 19%, respectively). Several genes involved in putative and known fungal virulence and general pathogenicity were identified. The results provide important information for future research on this fungal pathogen
author	SILVA,EVELYN VALDÉS,JORGE HOLMES,DAVID SHMARYAHU,AMIR VALENZUELA,PABLO D T
author_facet	SILVA,EVELYN VALDÉS,JORGE HOLMES,DAVID SHMARYAHU,AMIR VALENZUELA,PABLO D T
author_sort	SILVA,EVELYN
title	Generation and analysis of expressed sequence tags from Botrytis cinerea
title_short	Generation and analysis of expressed sequence tags from Botrytis cinerea
title_full	Generation and analysis of expressed sequence tags from Botrytis cinerea
title_fullStr	Generation and analysis of expressed sequence tags from Botrytis cinerea
title_full_unstemmed	Generation and analysis of expressed sequence tags from Botrytis cinerea
title_sort	generation and analysis of expressed sequence tags from botrytis cinerea
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200018
work_keys_str_mv	AT silvaevelyn generationandanalysisofexpressedsequencetagsfrombotrytiscinerea AT valdesjorge generationandanalysisofexpressedsequencetagsfrombotrytiscinerea AT holmesdavid generationandanalysisofexpressedsequencetagsfrombotrytiscinerea AT shmaryahuamir generationandanalysisofexpressedsequencetagsfrombotrytiscinerea AT valenzuelapablodt generationandanalysisofexpressedsequencetagsfrombotrytiscinerea
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Generation and analysis of expressed sequence tags from Botrytis cinerea

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