Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)

Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the triso...

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Autores principales:	CAVIEDES,PABLO, CAVIEDES,RAÚL, RAPOPORT,STANLEY I
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Trisomy 16 dorsal root ganglion calcium current tissue culture patch clamp Down syndrome
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009
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spelling	oai:scielo:S0716-976020060003000092014-01-24Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)CAVIEDES,PABLOCAVIEDES,RAÚLRAPOPORT,STANLEY I Trisomy 16 dorsal root ganglion calcium current tissue culture patch clamp Down syndrome Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the trisomy 16 fetal mouse, an animal model of the human condition. Cultured trisomic DRG neurons from human and mouse fetuses present faster rates of depolarization and repolarization in the action potential compared to normal controls and a shorter spike duration. Also, trisomy 16 brain and spinal cord tissue exhibit reduced acetylcholine secretion. Therefore, we decided to study Ca2+ currents in cultured DRG neurons from trisomy 16 and age-matched control mice, using the whole-cell patch-clamp technique. Trisomic neurons exhibited a 62% reduction in Ca2+ current amplitude and reduced voltage dependence of current activation at -30 and -20 mV levels. Also, trisomic neurons showed slower activation kinetics for Ca2+ currents, with up to 80% increase in time constant values. Kinetics of the inactivation phase were similar in both conditions. The results indicate that murine trisomy 16 alter Ca2+ currents, which may contribute to impaired cell function, including neurotransmitter release. These abnormalities also may alter neural development.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009en10.4067/S0716-97602006000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Trisomy 16 dorsal root ganglion calcium current tissue culture patch clamp Down syndrome
spellingShingle	Trisomy 16 dorsal root ganglion calcium current tissue culture patch clamp Down syndrome CAVIEDES,PABLO CAVIEDES,RAÚL RAPOPORT,STANLEY I Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
description	Down syndrome is determined by the presence of an extra copy of autosome 21 and is expressed by multiple abnormalities, with mental retardation being the most striking feature. The condition results in altered electrical membrane properties of fetal dorsal root ganglia (DRG) neurons, as in the trisomy 16 fetal mouse, an animal model of the human condition. Cultured trisomic DRG neurons from human and mouse fetuses present faster rates of depolarization and repolarization in the action potential compared to normal controls and a shorter spike duration. Also, trisomy 16 brain and spinal cord tissue exhibit reduced acetylcholine secretion. Therefore, we decided to study Ca2+ currents in cultured DRG neurons from trisomy 16 and age-matched control mice, using the whole-cell patch-clamp technique. Trisomic neurons exhibited a 62% reduction in Ca2+ current amplitude and reduced voltage dependence of current activation at -30 and -20 mV levels. Also, trisomic neurons showed slower activation kinetics for Ca2+ currents, with up to 80% increase in time constant values. Kinetics of the inactivation phase were similar in both conditions. The results indicate that murine trisomy 16 alter Ca2+ currents, which may contribute to impaired cell function, including neurotransmitter release. These abnormalities also may alter neural development.
author	CAVIEDES,PABLO CAVIEDES,RAÚL RAPOPORT,STANLEY I
author_facet	CAVIEDES,PABLO CAVIEDES,RAÚL RAPOPORT,STANLEY I
author_sort	CAVIEDES,PABLO
title	Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
title_short	Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
title_full	Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
title_fullStr	Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
title_full_unstemmed	Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)
title_sort	altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (down syndrome)
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300009
work_keys_str_mv	AT caviedespablo alteredcalciumcurrentsinculturedsensoryneuronsofnormalandtrisomy16mousefetusesananimalmodelforhumantrisomy21downsyndrome AT caviedesraul alteredcalciumcurrentsinculturedsensoryneuronsofnormalandtrisomy16mousefetusesananimalmodelforhumantrisomy21downsyndrome AT rapoportstanleyi alteredcalciumcurrentsinculturedsensoryneuronsofnormalandtrisomy16mousefetusesananimalmodelforhumantrisomy21downsyndrome
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Altered calcium currents in cultured sensory neurons of normal and trisomy 16 mouse fetuses, an animal model for human trisomy 21 (Down Syndrome)

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